Additional file 1. Primer sequences used for detection of Helicobacter spp. and F. gastrosuis and thermocycling conditions.

Additional file 3. BLAST identity percentage interval of the different Helicobacter spp. and F. gastrosuis sequences obtained.

Additional file 3. BLAST identity percentage interval of the different Helicobacter spp. and F. gastrosuis sequences obtained.

Additional file 1. Search strategy.

Additional file 1: Figure S1. Raman spectrum of CVD grown single-layer graphene. Figure S2. Averaged response from measurement replicates of a single gMTA chip. Figure S3. XPS peak fitting for PBASE and PBASE + aptamer samples. Figure S4. Blank samples measurements in PBS. Figure S5. Stability of gMTA measurements. Figure S6. Dopamine attomolar detection with short incubation time.

Additional file 1.

Additional file 2.

10β-Hydroxyestra-1,4-diene-3,17-dione (HEDD) is a natural product described as having neuroprotective activity. However, the cytotoxic properties of this quinol are barely studied. Thus, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed in six cell lines (MCF-7, T47-D, LNCaP, HepaRG, Caco-2 and NHDF). Additionally, an in vitro estrogenicity assay and a cell viability analysis together with in silico molecular docking studies were carried out in order to understand the potential mechanism of cytotoxicity. Computational predictions of its pharmacokinetic and...

Cognitive dysfunction is part of the broad spectrum of clinical manifestations in older untreated hereditary transthyretin amyloidosis patients with peripheral polyneuropathy. The objective of this study is to systematically explore cognitive dysfunction in ATTRV30M amyloidosis patients whose disease course was modified by liver transplant (LT). A series of 269 carriers of TTRVal30Met mutation treated with LT underwent a neuropsychological assessment. Clinical charts were reviewed to identify focal neurological episodes (FNEs), cognitive complaints and laboratory results....

Within the thymus, thymic epithelial cells (TECs) provide dedicated thymic stroma microenvironments for T cell development. Because TEC functionality is sensitive to aging and cytoablative therapies, unraveling the molecular elements that coordinate their thymopoietic role has fundamental and clinical implications. Particularly, the selection of CD4 T cells depends on interactions between TCRs expressed on T cell precursors and self-peptides:MHC II complexes presented by cortical TECs (cTECs). Although the macroautophagy/autophagy-lysosomal protein degradation pathway is implicated in...

Artigo de Rotura - Revista de Comunicação, Cultura e Artes ROTURA, 2 (2021): 41-48 eISSN: 2184-8661

Additional file 2. Nonparametric Kruskal–Wallis, Chi Square test applied to the three gastric zones comparing the different variables in pigs.

Additional file 2. Nonparametric Kruskal–Wallis, Chi Square test applied to the three gastric zones comparing the different variables in pigs.

Additional file 4. Number of Helicobacter spp. and F. gastrosuis DNA positive samples associated with gastritis score per pig gastric zone.

Additional file 1: Figure S1. Gating strategy to detect CD69+ or CD25+ cells following sAg-mediated activation. E6.1-CD6WT and E6.1-CD6Dcyt cells were cultured for 24 h in the presence of unloaded or sAg-loaded Raji cells, and expression of CD69 and CD25 was assessed by flow cytometry. (a) The gate was defined based on unstained E6.1 cells, and positivity is represented in the right half of dot plots of FSC vs. marker expression. (b) Representative dot plots...

Additional file 1: Figure S1. Raman spectrum of CVD grown single-layer graphene. Figure S2. Averaged response from measurement replicates of a single gMTA chip. Figure S3. XPS peak fitting for PBASE and PBASE + aptamer samples. Figure S4. Blank samples measurements in PBS. Figure S5. Stability of gMTA measurements. Figure S6. Dopamine attomolar detection with short incubation time.

Both vitamin A and E support female reproduction and embryonic development. These vitamins have been associated with decreased fertility or failure to end the pregnancy in animals. An observational study was conducted on follicular fluid (FF) samples to determine the concentrations of fat-soluble vitamins of women undergoing in vitro fertilization and its correlation with assisted reproductive technology characteristics and pregnancy outcomes. Moreover, the effects of all-trans-retinoic acid (atRA) and alpha-tocopherol on granulosa cell viability, apoptosis,...

Supplementary Material 1

Supplementary Material 2

Additional file 1. Additional methods (definitions, additional methods, statistical analyses and ethics), Additional tables (Tables S1–S8) and Additional figures (Figs. S1–S2).

Additional file 1. Additional methods (definitions, additional methods, statistical analyses and ethics), Additional tables (Tables S1–S8) and Additional figures (Figs. S1–S2).

Additional file 1: Figure S1. Gating strategy to detect CD69+ or CD25+ cells following sAg-mediated activation. E6.1-CD6WT and E6.1-CD6Dcyt cells were cultured for 24 h in the presence of unloaded or sAg-loaded Raji cells, and expression of CD69 and CD25 was assessed by flow cytometry. (a) The gate was defined based on unstained E6.1 cells, and positivity is represented in the right half of dot plots of FSC vs. marker expression. (b) Representative dot plots...

Both vitamin A and E support female reproduction and embryonic development. These vitamins have been associated with decreased fertility or failure to end the pregnancy in animals. An observational study was conducted on follicular fluid (FF) samples to determine the concentrations of fat-soluble vitamins of women undergoing in vitro fertilization and its correlation with assisted reproductive technology characteristics and pregnancy outcomes. Moreover, the effects of all-trans-retinoic acid (atRA) and alpha-tocopherol on granulosa cell viability, apoptosis,...

Wave climatologies from historical and projected simulations of the ACCESS1.0, MIROC5 and CNRM-CM5 Global Circulation Models (GCM) were sourced from the Coordinated Ocean Wave Climate Project (COWCLIP) and downscaled using the SWAN wave model. Biases between GCM's historical simulations and a regional hindcast were assessed, and the two best-performing models (ACCESS1.0, MIROC5) had their projections analysed. A general increase in wave height and period was observed along the south/west, together with a decrease in Hs...

Additional file 2: Supplementary Table 1. Baseline features of the extension BODI cohort recruited for the analysis of the association between damage accrual and Heath related quality of life (n=147).