2,576 Works

Landscape of RNA editing reveals new insights into the dynamic gene regulation of spermatogenesis

Xiaodan Wang, Xiaolong Wu, Zhenshuo Zhu, Hao Li, Tongtong Li, Qun Li, Peng Zhang, Leijie Li, Dongxue Che, Xia Xiao, Tong Liu, Jinlian Hua & Mingzhi Liao
Spermatogenesis is an important physiological process associated with male infertility. As a kind of post-transcriptional regulation, RNA editings (REs) change the genetic information at the mRNA level. But whether there are REs and what’s the role of REs during the process are still unclear. In this study, we integrated published RNA-Seq datasets and established a landscape of RNA REs during the development of mouse spermatogenesis. Totally, 7530 editing sites occurred in 2012 genes among all...

Identification of Volatile Components in Tea Infusions by Headspace–Programmed Temperature Vaporization–Gas Chromatography–Mass Spectrometry (HS–PTV–GC–MS) with Chemometrics

Yan Zhou, Jie Yu, Liang Wang, Da Wu, Dawei Qi, Yunfei Sha & Baizhan Liu
In this work, the two-step headspace (HS) technique was applied to determine the actual volatile compositions of tea infusions. In order to eliminate the influence of water vapor to the gas chromatography-mass spectrometry (GC-MS) analysis and enable the capability to determine the volatile compounds in water base samples, a programed temperature vaporizer (PTV) was employed for the additional pre-concentration of thee analytes. While using the reported method, the volatile compounds were retained by the absorbent...

Iodine mediated synthesis of diaryl diselenides using SeO2 as a selenium source

Dileep Kommula, Qing Li, Siyang Ning, Wujun Liu, Qian Wang & Zongbao K. Zhao
A simple, eco-friendly and convenient procedure has been developed for the synthesis of diphenyl diselinides from readily available aryl boronic acids by reaction with SeO2 in the presence of iodine under simple conditions. The methodology is reported about 19 examples and applicable to a broad scope of aryl boronic acids containing electron-rich and electron-poor substituents. Apart from being milder and environmentally benign conditions, this synthetic protocol comprises a novel, reliable methodology to give good to...

Gain of function mutant p53 protein activates AKT through the Rac1 signaling to promote tumorigenesis

Xuetian Yue, Fangnan Wu, Yanchen Li, Juan Liu, Michael Boateng, Kranthi Mandava, Cen Zhang, Zhaohui Feng, Jimin Gao & Wenwei Hu
Tumor suppressor p53 is the most frequently mutated gene in human cancer. Mutant p53 (mutp53) not only loses the tumor suppressive activity of wild type p53, but often gains new oncogenic activities to promote tumorigenesis, defined as mutp53 gain of function (GOF). While the concept of mutp53 GOF is well-established, its underlying mechanism is not well-understood. AKT has been suggested to be activated by mutp53 and contribute to mutp53 GOF, but its underlying mechanism is...

Stabilized diverse HIV-1 envelope trimers for vaccine design

Qian Wang, Bingting Ma, Qingtai Liang, Angqi Zhu, Hua Wang, Lili Fu, Xiaoxu Han, Xuanling Shi, Ye Xiang, Hong Shang & Linqi Zhang
One of the major goals in HIV-1 vaccine development is to achieve properly folded and stabilized envelope glycoprotein (Env) trimers that mimic the native Env on the mature virion. Here, we design and characterize uncleaved prefusion-optimized (UFO) trimers for 12 Envs currently circulating in China. Biochemical and biophysical characterization of these UFO trimers identified two subtype B/Bʹ Envs, CNE6 and MG13, which exhibited the highest trimer content and stability at a level comparable to the...

Stabilized diverse HIV-1 envelope trimers for vaccine design

Qian Wang, Bingting Ma, Qingtai Liang, Angqi Zhu, Hua Wang, Lili Fu, Xiaoxu Han, Xuanling Shi, Ye Xiang, Hong Shang & Linqi Zhang
One of the major goals in HIV-1 vaccine development is to achieve properly folded and stabilized envelope glycoprotein (Env) trimers that mimic the native Env on the mature virion. Here, we design and characterize uncleaved prefusion-optimized (UFO) trimers for 12 Envs currently circulating in China. Biochemical and biophysical characterization of these UFO trimers identified two subtype B/Bʹ Envs, CNE6 and MG13, which exhibited the highest trimer content and stability at a level comparable to the...

Emerging colistin resistance in Salmonella enterica serovar Newport isolates from human infections

Mohammed Elbediwi, Hang Pan, Silpak Biswas, Yan Li & Min Yue
Worldwide emergence of Salmonella enterica serovar Newport (S. Newport) infection in humans, in parallel with a significant increasing prevalence of antimicrobial resistance (AR), is a serious public health concern. However, the prevalence of S. Newport resistance in China remains largely unknown. A retrospective study of 287 S. Newport clinical isolates collected during 1997–2018 was undertaken for characterization of AR profiles using the micro-dilution assay. We found a recent emergence of colistin resistance in four Chinese...

Investigation of Pb (II) and Cd (II) removal from aqueous solution by organic-modified xonotlite

Wei Zhou, Junliang Xin & Wenqing Tang
In this work, a novel xonotlite nanocomposite was synthesised with abandoned eggshell by hydrothermal method, and organic-modified with toluene diisocyanate (TDI) for the removal of Pb(II) and Cd(II) from wastewater. The xonotlite and organic-modified xonotlite were characterised by X-ray diffraction (XRD), scanning electron microscopy (SEM), Brunner-Emmet-Teller (BET) and Fourier transform infrared spectroscopy (FTIR) methods. The effect of solution pH, contact time, adsorbent dose, and initial concentrations were also investigated. The organic-modified xonotlite was found to...

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

Xinjin Liu, Jiangpeng Feng, Qiuhan Zhang, Dong Guo, Lu Zhang, Tao Suo, Wenjia Hu, Ming Guo, Xin Wang, Zhixiang Huang, Yong Xiong, Guozhong Chen, Yu Chen & Ke Lan
Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method. In our recent study, we explored the feasibility of droplet digital PCR (ddPCR) for clinical SARS-CoV-2 nucleic acid detection compared with qRT-PCR using the same primer/probe sets issued by Chinese Center for Disease Control and Prevention (CDC) targeting viral ORF1ab or N gene, which showed that...

ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens

Tao Suo, Xinjin Liu, Jiangpeng Feng, Ming Guo, Wenjia Hu, Dong Guo, Hafiz Ullah, Yang Yang, Qiuhan Zhang, Xin Wang, Muhanmmad Sajid, Zhixiang Huang, Liping Deng, Tielong Chen, Fang Liu, Ke Xu, Yuan Liu, Qi Zhang, Yingle Liu, Yong Xiong, Guozhong Chen, Ke Lan & Yu Chen
Quantitative real time PCR (RT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, due to the low viral load specimens and the limitations of RT-PCR, significant numbers of false negative reports are inevitable, which results in failure to timely diagnose, cut off transmission, and assess discharge criteria. To improve this situation, an optimized droplet digital PCR (ddPCR) was used for detection of SARS-CoV-2, which showed that the limit of detection...

Stochastic Tree Search for Estimating Optimal Dynamic Treatment Regimes

Yilun Sun & Lu Wang
A dynamic treatment regime (DTR) is a sequence of decision rules that adapt to the time-varying states of an individual. Black-box learning methods have shown great potential in predicting the optimal treatments; however, the resulting DTRs lack interpretability, which is of paramount importance for medical experts to understand and implement. We present a stochastic tree-based reinforcement learning (ST-RL) method for estimating optimal DTRs in a multistage multitreatment setting with data from either randomized trials or...

SARS-CoV-2 neutralizing antibody responses are more robust in patients with severe disease

Pengfei Wang, Lihong Liu, Manoj S. Nair, Michael T. Yin, Yang Luo, Qian Wang, Ting Yuan, Kanako Mori, Axel Guzman Solis, Masahiro Yamashita, Ankur Garg, Lawrence J. Purpura, Justin C. Laracy, Jian Yu, Leemor Joshua-Tor, Joseph Sodroski, Yaoxing Huang & David D. Ho
We studied plasma antibody responses of 35 patients about 1 month after SARS-CoV-2 infection. Titers of antibodies binding to the viral nucleocapsid and spike proteins were significantly higher in patients with severe disease. Likewise, mean antibody neutralization titers against SARS-CoV-2 pseudovirus and live virus were higher in the sicker patients, by ∼5-fold and ∼7-fold, respectively. These findings have important implications for those pursuing plasma therapy, isolation of neutralizing monoclonal antibodies, and determinants of immunity.

Identification of a promoter polymorphism affecting GPAT3 gene expression that is likely related to intramuscular fat content in pigs

Cai Ma, Yi Sun, Jiying Wang, Li Kang & Yunliang Jiang
The intramuscular fat content (IMF) is an economically important trait in pigs and the Laiwu pig is famous for its excessively extremely high level of IMF. Our previous transcriptome study revealed that the dynamic expression of glycerol-phosphate acyltransferase 3 (GPAT3) is consistent with changes in the IMF of Laiwu pigs. In this study, we further analyzed the expression and polymorphism of GPAT3 in its promoter region. The results indicated that the expression of GPAT3 increased...

Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone of K. pneumoniae: An epidemiological, genomic and functional study

Ping Li, Qiqiang Liang, Wugao Liu, Beiwen Zheng, Lizhang Liu, Wei Wang, Zhijiang Xu, Man Huang & Youjun Feng
Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

Sustained mTORC1 activity during palbociclib-induced growth arrest triggers senescence in ER+ breast cancer cells

Reeja S. Maskey, Fang Wang, Elyssa Lehman, Yiqun Wang, Natasha Emmanuel, Wenyan Zhong, Guixian Jin, Robert T. Abraham, Kim T. Arndt, Jeremy S. Myers & Anthony Mazurek
Palbociclib, a selective CDK4/6 kinase inhibitor, is approved in combination with endocrine therapies for the treatment of advanced estrogen receptor positive (ER+) breast cancer. In pre-clinical cancer models, CDK4/6 inhibitors act primarily as cytostatic agents. In two commonly studied ER+ breast cancer cell lines (MCF7 and T47D), CDK4/6 inhibition drives G1-phase arrest and the acquisition of a senescent-like phenotype, both of which are reversible upon palbociclib withdrawal (incomplete senescence). Here we identify an ER+ breast...

Additional file 1 of Effectiveness of electroacupuncture for thin endometrium in infertile women: study protocol for a single-blind, randomized controlled trial

Fangyuan Li, Hua Lu, Xinxin Wang, Qi Zhang, Qianchen Liu & Tong Wang
Additional file 1.

Establishing a high sensitivity detection method for SARS-CoV-2 IgM/IgG and developing a clinical application of this method

Chunyan Zhang, Lei Zhou, Hao Liu, Sibing Zhang, Yaping Tian, Junli Huo, Fei Li, Yao Zhang, Bo Wei, Dan Xu, Jinwei Hu, Jiayi Wang, Yuxuan Cheng, Wenjie Shi, Xiuli Xu, Jianping Zhou, Peipei Sang, Xudong Tan, Weiwei Wang, Minjie Zhang, Bin Wang, Yujun Zhou, Kan Zhang & Kunlun He
COVID-19 is caused by SARS-CoV-2 infection and was initially discovered in Wuhan. This outbreak quickly spread all over China and then to more than 20 other countries. SARS-CoV-2 fluorescent microsphere immunochromatographic test strips were prepared by the combination of time-resolved fluorescence immunoassay with a lateral flow assay. The analytical performance and clinical evaluation of this testing method was done and the clinical significance of the testing method was verified. The LLOD of SARS-CoV-2 antibody IgG...

Effect of spray properties on aerosol scavenging efficiency with water mist

Hui Liang, Qian Zhou, Nejdet Erkan & Shunichi Suzuki
In the foreseen decommissioning plans of damaged Fukushima Daiichi reactors, submicron aerosol particles will be generated when retrieving fuel debris from reactor buildings by cutting it into small pieces. A multi-nozzle spray system will be used to scavenge these aerosol particles inside the primary containment vessel. To improve the aerosol removal efficiency of the multi-nozzle spray system, it is now necessary to study the aerosol removal performance by different single spray nozzles with different spray...

Trypanosoma evansi evades host innate immunity by releasing extracellular vesicles to activate TLR2-AKT signaling pathway

Ran Wei, Xin Li, Xiaocen Wang, Nan Zhang, Yuru Wang, Xichen Zhang, Pengtao Gong & Jianhua Li
Surra, one of the most important animal diseases with economic consequences in Asia and South America, is caused by Trypanosoma evansi. However, the mechanism of immune evasion by T. evansi has not been extensively studied. In the present study, T. evansi extracellular vesicles (TeEVs) were characterized and the role of TeEVs in T. evansi infection were examined. The results showed that T. evansi and TeEVs could activate TLR2-AKT pathway to inhibit the secretions of IL-12p40,...

Cross-cultural adaptation, reliability, and validity of the Chinese version of the Acceptance and Action Questionnaire-Adult Hearing Loss

Ying Yang, Yulu Liu, Yanan Xiao, Lianchi Zhang, Yan Li, Hong Zhu & Jin Bian
Few mental health assessment tools are available for people with hearing loss (HL) in China. The Acceptance and Action Questionnaire-Adult Hearing Loss (AAQ-AHL) has been specifically designed to assess psychological inflexibility in adults with HL and may help assess mental health status promptly for targeted psychological interventions. The study aimed to investigate the cross-cultural validity and reliability of the Chinese version of the AAQ-AHL to assess its applicability to teenagers and adults with HL in...

Additional file 1 of Effect of preoperative CT angiography examination on the clinical outcome of patients with BMI ≥ 25.0 kg/m2 undergoing laparoscopic gastrectomy: study protocol for a multicentre randomized controlled trial

Cheng Meng, Shougen Cao, Xiaodong Liu, Leping Li, Qingsi He, Lijian Xia, Lixin Jiang, Xianqun Chu, Xinjian Wang, Hao Wang, Xizeng Hui, Zuocheng Sun, Shusheng Huang, Quanhong Duan, Daogui Yang, Huanhu Zhang, Yulong Tian, Zequn Li & Yanbing Zhou
Additional file 1. Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 checklist.

Comparison of the accelerating effect of graphene oxide and graphene on anaerobic transformation of bisphenol F by Pseudomonas sp. LS

Hong Lu, Jingyi Li, Ze Fu, Xiaolei Wang, Jiti Zhou & Jing Wang
It was found that bisphenol F (BPF) could be anaerobically transformed to 4,4-dihydroxybenzophenone using nitrate as an electron acceptor by Pseudomonas sp. LS. However, BPF removal was a slow process under anaerobic conditions. In the present study, effects of graphene oxide (GO) and graphene on the anaerobic transformation of BPF were studied in detail. Results showed that GO (2–10 mg/L) and graphene (2–20 mg/L) could increase the anaerobic biotransformation rate of BPF. For GO-mediated system,...

Risk of Ophthalmic Adverse Events in Patients Treated with Immune Checkpoint Inhibitor Regimens: A Systematic Review and Meta-analysis

Yan-Li Hou, Di-Ya Wang, Jie-Xuan Hu, Ru-Yue Tian, Wei Wang, Qiang Su, Hongyang Li & Yan-Ling Wang
Immune checkpoint inhibitors (ICIs) -induced adverse events (AEs) have been reported affecting almost all human organs. However, studies about ocular AEs are few. A meta-analysis was performed to evaluate the risks of ICI-related ophthalmic AEs compare to chemotherapy. Methods: Eligible studies were selected from phase II/III randomized controlled trials investigating ICIs. The data were analyzed by R software and Stata. Odds ratio of treatment-related AE (trAEs) and nonspecific ophthalmic trAEs (NS-trAEs) were lower for PD-1/PD-L1...

Functional redundancy changes along a drought stress gradient for the shift of selection effect to complementarity effect in experimental plant communities

Song Gao, Xiaoyan Wang, Tong Chen, Qiaodi Yan, Mengchun Shen, Jiang Wang & Feihai Yu
Species interactions alter with environmental change, which influences the relationship between biodiversity and ecosystem functioning (BEF). How BEF relationships change with environments requires knowledge of the changes of functional attributes in individual species in response to environmental change. Here, we assembled experimental plant communities with different species richness (1, 2, 4, 8, and 16) and exposed them to a drought stress gradient (control, moderate and intensive drought stress). Functional redundancy decreased with increasing drought stress...

Reproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts

Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Jing Liu, Mikkel Meyer Andersen, Jacob Tfelt-Hansen & Niels Morling
The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson’s...

Registration Year

  • 2023
  • 2022
  • 2021
  • 2020
  • 2019
  • 2018
  • 2017

Resource Types

  • Text


  • Chinese PLA General Hospital
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Fudan University
  • Zhejiang University
  • Capital Medical University
  • Sichuan University
  • Sun Yat-sen University
  • Huazhong University of Science and Technology
  • Chinese Academy of Sciences