Pancreatic cancer is one of the leading causes of cancer-related deaths worldwide. Poor prognosis and low survival rates have driven the development of novel therapeutic strategies. Nanosecond pulsed electric field has emerged as a novel, minimal invasive and non-thermal treatment for solid tumors. It is of great significance to study the combination therapy of nsPEF and other treatment strategies for pancreatic cancer. We developed neutrophil membrane-wrapped liposomal nanoparticles loaded with gemcitabine (NE/Lip-GEM) and investigated their...

Supplemental document with additional data, simulation results, and figures.

Supplement 1

Resembling acute promyelocytic leukemia (APL) is a unique subtype of APL who sharing clinical, morphological, and immunophenotypic features with typical APL, but lacking evidence of PML-RARA fusion gene and usually insensitive to arsenic trioxide (ATO) and all-trans retinoic acid (ATRA). For years, RARA, RARB and RARG rearrangement were found in resembling APL continually. The confirmed partner genes of RARG rearrangement included CPSF6, NUP98, NPM1, PML, and HNRNPC. These patients were a group of resembling APL...

In order to overcome the pandemic of COVID-19, messenger RNA (mRNA)-based vaccine has been extensively researched as a rapid and versatile strategy. Herein, we described the immunogenicity of mRNA-based vaccines for Beta and the most recent Omicron variants. The homologous mRNA-Beta and mRNA-Omicron and heterologous Ad5-nCoV plus mRNA vaccine exhibited high-level cross-reactive neutralization for Beta, original, Delta, and Omicron variants. It indicated that the COVID-19 mRNA vaccines have great potential in the clinical use against...

Macroautophagy/autophagy, a highly conserved catabolic pathway that maintains proper cellular homeostasis is stringently regulated by numerous autophagy-related (Atg) proteins. Many studies have investigated autophagy regulation at the transcriptional level; however, relatively little is known about translational control. Here, we report the upstream open reading frame (uORF)-mediated translational control of multiple Atg proteins in Saccharomyces cerevisiae and in human cells. The translation of several essential autophagy regulators in yeast, including Atg13, is suppressed by canonical uORFs...

Additional file 1: Details of outcomes. Table S1. The UDI, definition and measurements of covariates. Table S2. Characteristics of the participants included or excluded in the study. Table S3. Distributions of the annual average exposures among 398,993 participants. Table S4. Associations between air pollution and risk of cause-specific mortality. Table S5. Results of 2-pollutant models. Table S6. Results of sensitivity analyses in the model including traffic noise (n = 393,515). Table S7. Results of sensitivity...

Additional file 4: Table S1. Synthetic lethal interactive pairs and chemical inhibitors involving SWI/SNF members.

Additional file 1. The details of algorithm design, network training and testing.

Additional file 1. Analysis and interpretation process of fetal ES data.

Additional file 1: Fig. S1. A schematic showing the experimental design, milk feeding, and fecal sample collection of the two trials.

Additional file 2: Fig. S2. Heatmap of the ASVs associated with diarrheic status in stage 4 of trial 1. The ASVs were identified based on fold change and analysis using LEfSe or DESeq2.

Additional file 5: Fig. S4. Heatmap of the ASVs that identified to be diarrheic status transition-associated in trial 2. The ASVs were identified based on fold change and analysis using LEfSe or DESeq2.

Additional file 1.

Additional file 2. Searching strategies.

Additional file 1: Figure S1. (A) Representative images of dissected tumors across the different treatment groups. (B-C) PCNA protein expression levels were selected as indices of tumor tissue proliferation for immunohistochemical detection and analysis. (D-E) TUNEL experiment of tumor tissues in different treatment groups. Figure S2. Hematoxylin and eosin staining tests (Figure S2A) and serum biochemical indices, including albumin, alanine aminotransferase, creatinine, and urea levels,(Figure S2B-E) showed that there was no significant histological evidence of...

Additional file 1. The tables of the filtering parameters setting using Prinseq-lite software, primers and probes used to detect co-infection respiratory viruses, type analysis of common virus-related sequence viral in MP positive and MP negative (TTV has been removed). Table S1: The filtering parameters setting using Prinseq-lite software. Table S2: Primers and probes used to detect co-infection respiratory viruses. Table S3: Type analysis of common virus-related sequence viral in MP positive and MP negative (TTV...

Additional file 2: Table S2. Comparison of clinical outcomes between single first-line and repeated first-line treatment subgroups in rituximab cohort

A wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-centre retrospective study was conducted in...

Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). There are no available drugs due to the emergence of variant of PRV. Dihydromyricetin (DMY) is a flavonoid extracted from Ampelopsis grossedentata (A. grossedentata), which has a variety of pharmacological activities. In this study, we aim to investigate the in vitro anti-PRV activity of DMY extracted from A. grossedentata. MTT assay was used to detect the cytotoxicity and antiviral activity of DMY. The...

Additional file 2. PRECIS-2 scores of chaiqin chengqi decoction for predicted moderately severe and severe acute pancreatitis (CAP trial) domains.

Additional file 1. Table S1. Clinical and laboratory data in septic patients and healthy donors.

With titanium wire as a titanium source and a fiber substrate, titania nanostrips (TiO2NSs) were in situ grown on the fiber surface by electrochemical anodization and used as supporting templates for subsequent uniform deposition of polar glucose via hydrothermal reaction. After annealing, controllable in situ derivation of TiO2NSs-templated carbonaceous coating (TiO2@CNSs) was achieved on the Ti fiber substrate. Coupled to high-performance liquid chromatography with UV detection, the extraction performance was investigated using representative aromatic compounds...

With the extensive application of titanium dioxide nanoparticles (TiO2 NPs), their impacts on calcium homeostasis have aroused extensive attention from scholars. However, there are still some controversies in relevant reports. Therefore, a systematic review was performed followed by a meta-analysis to explore whether TiO2 NPs could induce the imbalance in calcium homeostasis in vivo and in vitro through Revman5.4 and Stata15.0 in this research. Fourteen studies were included through detailed database retrieval and literature screening....

Two new nor-triterpenoids ganodrenol A (1), B (2), and a new natural product ganodrenol C (3), along with three known nor-triterpenoids (4–6) were isolated from the fruiting bodies of Ganoderma lucidum. The chemical structures of these isolates were determined by 1 D and 2 D NMR, HRESIMS, and X-ray crystallography analysis. The inhibitory effects of isolated triterpenoids (1–6) against FAAH were evaluated by an in vitro assay, and compound 4 showed an inhibition rate of...