107 Works

Upregulated HMGB1 levels in maternal–fetal interface of patients with unexplained recurrent spontaneous abortion from different sources

Jing Wang, Damin Zhu, Jiaqian Yin, Cong Ma, Xiaoqing Peng, Huijuan Zou, Yunxia Cao & Xiaofeng Xu
To investigate the expression and sources of high mobility group box 1 (HMGB1) protein in the maternal–fetal interface of patients with unexplained recurrent spontaneous abortion (URSA), and further to verify the role of HMGB1 in the etiology of URSA. 55 women at early pregnancy with URSA and 55 women undergoing selective termination of normal early pregnancy as control were included. The abortion tissues including villi and decidua were collected. The expression of HMGB1, CD45, CK7,...

Supplemental Material, sj-doc-1-gsj-10.1177_21925682211005732 - Clinicopathological and Prognostic Characteristics in Spinal Chondroblastomas: A Pooled Analysis of Individual Patient Data From a Single Institute and 27 Studies

Bo-Wen Zheng, Wei Huang, Fu-Sheng Liu, Tao-Lan Zhang, Xiao-Bin Wang, Jing Li, Guo-Hua Lv, Yi-Guo Yan & Ming-Xiang Zou
Supplemental Material, sj-doc-1-gsj-10.1177_21925682211005732 for Clinicopathological and Prognostic Characteristics in Spinal Chondroblastomas: A Pooled Analysis of Individual Patient Data From a Single Institute and 27 Studies by Bo-Wen Zheng, Wei Huang, Fu-Sheng Liu, Tao-Lan Zhang, Xiao-Bin Wang, Jing Li, Guo-Hua Lv, Yi-Guo Yan and Ming-Xiang Zou in Global Spine Journal

High Child-Pugh and CRUB65 scores predict mortality of decompensated cirrhosis patients with COVID-19: A 23-center, retrospective study

Yong Xiao, Dongwen Wu, Xiao Shi, Shuzhong Liu, Xudong Hu, Chenliang Zhou, Xia Tian, Huimin Liu, Hui Long, Zhihong Li, Ji Wang, Tao Tan, Ying Xu, Bitao Chen, Ting Liu, Heng Zhang, Shihua Zheng, Shunlin Hu, Jun Song, Jie Tang, Jichun Song, Zhengwei Cheng, Weitian Xu, Yongxiang Shen, Wenhu Yu … & Mingkai Chen
Background: COVID-19 has rapidly become a major health emergency worldwide. The characteristic, outcome, and risk factor of COVID-19 in patients with decompensated cirrhosis remain unclear. Methods: Medical records were collected from 23 Chinese hospitals. Patients with decompensated cirrhosis and age- and sex-matched non-liver disease patients were enrolled with 1:4 ratio using stratified sampling. Results: There were more comorbidities with higher Chalson Complication Index (p < 0.001), higher proportion of patients having gastrointestinal bleeding, jaundice, ascites,...

High Child-Pugh and CRUB65 scores predict mortality of decompensated cirrhosis patients with COVID-19: A 23-center, retrospective study

Yong Xiao, Dongwen Wu, Xiao Shi, Shuzhong Liu, Xudong Hu, Chenliang Zhou, Xia Tian, Huimin Liu, Hui Long, Zhihong Li, Ji Wang, Tao Tan, Ying Xu, Bitao Chen, Ting Liu, Heng Zhang, Shihua Zheng, Shunlin Hu, Jun Song, Jie Tang, Jichun Song, Zhengwei Cheng, Weitian Xu, Yongxiang Shen, Wenhu Yu … & Mingkai Chen
Background: COVID-19 has rapidly become a major health emergency worldwide. The characteristic, outcome, and risk factor of COVID-19 in patients with decompensated cirrhosis remain unclear. Methods: Medical records were collected from 23 Chinese hospitals. Patients with decompensated cirrhosis and age- and sex-matched non-liver disease patients were enrolled with 1:4 ratio using stratified sampling. Results: There were more comorbidities with higher Chalson Complication Index (p < 0.001), higher proportion of patients having gastrointestinal bleeding, jaundice, ascites,...

Identification of the structural features of quinazoline derivatives as EGFR inhibitors using 3D-QSAR modeling, molecular docking, molecular dynamics simulations and free energy calculations

Fangfang Wang, Wei Yang, Hongping Liu & Bo Zhou
Epidermal growth factor receptor (EGFR) is a promising target for the treatment of different types of malignant tumors. Therefore, a combined molecular modeling study was performed on a series of quinazoline derivatives as EGFR inhibitors. The optimum ligand-based CoMFA and CoMSIA models showed reliable and satisfactory predictability (with R2cv=0.681, R2ncv=0.844, R2pred=0.8702 and R2cv=0.643, R2ncv=0.874, R2pred=0.6423). The derived contour maps provide structural features to improve inhibitory activity. Furthermore, the contour maps, molecular docking, and molecular dynamics...

Folate-functionalized SMMC-7721 liver cancer cell membrane-cloaked paclitaxel nanocrystals for targeted chemotherapy of hepatoma

Wenwen Shen, Shuke Ge, Xiaoyao Liu, Qi Yu, Xue Jiang, Qian Wu, YuChen Tian, Yu Gao, Ying Liu & Chao Wu
In this study, we prepared a folic acid-functionalized SMMC-7721 liver cancer cell membrane (CM)-encapsulated paclitaxel nanocrystals system (FCPN) for hepatoma treatment. Transmission electron microscopy (TEM) characterization showed that FCPN was irregular spherical shapes with a particle size larger than 200 nm and a coated thickness of approximately 20 nm. In an in vitro release experiment, FCPN indicated a slowly release effect of paclitaxel (PTX). Cell experiments demonstrated that FCPN was taken up by SMMC-7721 cells...

Heterologous expression and enzymatic identification of two novel soluble pyridine nucleotide transhydrogenases from Acidobacteria bacterium KBS 146 and Nocardia jiangxiensis

Zhengyu Cao, Rui Meng, Peng Wang & Guoping Zhu
Soluble pyridine nucleotide transhydrogenase (STH), catalyzes a reversible hydrogen transfer between NADH and NADPH, is widely utilized in cofactor engineering. In the present study, we expanded the distribution range of STH to more unreported bacteria. Meanwhile, STH was mainly found in α, γ, δ-proteobacteria, acidobacteria, actinobacteria and planctomycetes. The enzymological properties of two novel STHs from Acidobacteria bacterium KBS 146 (AbSTH) and Nocardia jiangxiensis (NjSTH) were characterized. The optimum temperature and pH of AbSTH and...

Buyang Huanwu decoction improves neural recovery after spinal cord injury in rats through the mTOR signaling pathway and autophagy

Ying Nie, Yujie Fan, Xi Zhang, Xiaosong Li, Jian Yin, Meili Li, Zhaoyong Hu, Liang Li & Xiaoye Wang
Spinal cord injury (SCI) refers to the interruption of the tracts inside the spinal cord caused by various factors. The repair of damaged axons has always been a difficult point in clinical treatment and neuroscience research. The treatment of SCI with Buyang huanwu decoction (BYHWD), a well-known recipe for invigorating Qi (a vital force forming part of any living entity in traditional Chinese culture) and promoting blood circulation, shows a good effect. The rubrospinal tract...

Hepatitis B virus middle surface antigen loss promotes clinical variant persistence in mouse models

Junyu Lin, Jing Li, Peilin Xie, Yue Han, Demin Yu, Jia Chen & Xinxin Zhang
Hepatitis B virus (HBV) middle surface antigen (MHBs) mutation or deletion occurs in patients with chronic HBV infection. However, the functional role of MHBs in HBV infection is still an enigma. Here, we reported that 7.33% (11/150) isolates of CHB patients had MHBs start codon mutations compared with 0.00% (0/146) in acute hepatitis B (AHB) patients. Interestingly, MHBs loss accounted for 11.88% (126/1061) isolates from NCBI GenBank, compared with 0.09% (1/1061) and 0.00% (0/1061) for...

CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression

Xuan Li, Kai-Bin Yang, Wei Chen, Jia Mai, Xiao-Qi Wu, Ting Sun, Rui-Yan Wu, Lin Jiao, Dan-Dan Li, Jiao Ji, Hai-Liang Zhang, Yan Yu, Yu-Hong Chen, Gong-Kan Feng, Rong Deng, Jun-Dong Li & Xiao-Feng Zhu
Macroautophagy/autophagy plays an important role during the development of human cancer. BECN1 (beclin 1), a core player in autophagy regulation, is downregulated in many kinds of malignancy. The underlying mechanism, however, has not been fully illuminated. Here, we found that CUL3 (cullin 3), an E3 ubiquitin ligase, could interact with BECN1 and promote the K48-linked ubiquitination and degradation of this protein; In addition, CUL3 led to a decrease in autophagic activity through downregulating BECN1. We...

Discovery of novel serum metabolic biomarkers in patients with polycystic ovarian syndrome and premature ovarian failure

Jiying Chen, Qinger Zhou, Yonggang Zhang, Wenqing Tan, Hanchao Gao, Liying Zhou, Shuixiu Xiao, Jinhua Gao, Jing Li & Zhiying Zhu
Several widely recognized metabolites play a role in regulating the pathophysiological processes of various disorders. Nonetheless, the lack of effective biomarkers for the early diagnosis of polycystic ovarian syndrome (PCOS) and premature ovarian failure (POF) has led to the discovery of serum-based metabolic biomarkers for these disorders. We aimed to identify various differentially expressed metabolites (DEMs) through serum-based metabolic profiling in patients with PCOS and POF and in healthy individuals by using liquid chromatography–mass spectrometry...

Ubiquilin 1 suppresses the cancer stem cell–like traits of non-small cell lung cancer cells by regulating reactive oxygen species homeostasis

Ting Liu, Qianqian Ma, Wenjie Li, Yan Hu, Jun Yang & Qi Yao
Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC are still lacking key elements. In the current study, we found that reductive redox states and ROS levels were suppressed in non-adherent spheres formed by non-small cell lung cancer (NSCLC) cells, which were confirmed to...

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF

Ying Xu, Wen Xu, Wei Liu, Gaofeng Chen, Shili Jiang, Jiamei Chen, Xun Jian, Hua Zhang, Ping Liu & Yongping Mu
Our previous studies indicated that Yiguanjian decoction (YGJ) has an anti-hepatic-fibrosis effect and could regulate macrophage status. To elucidate the mechanism of YGJ in regulating macrophages. Liver cirrhosis was induced by CCl4 for 12 weeks combined with 2-acetylaminofluorene (2-AAF) for the last 4 weeks in male Wistar rats. YGJ (3.56 mg/kg) orally administered in the last 4 weeks, and SORA (1 mg/kg) as control. In vitro, RAW264.7 cells were treated with lipopolysaccharides (LPSs) to induce...

Prevalence and predictors of post-traumatic stress disorder in patients with cured coronavirus disease 2019 (COVID-19) one month post-discharge

Yumeng Ju, Jin Liu, Roger M. K. Ng, Bangshan Liu, Mi Wang, Wentao Chen, Mei Huang, Aiping Yang, Kongliang Shu, Yun Zhou, Li Zhang, Mei Liao, Jiyang Liu & Yan Zhang
Background: Coronavirus disease 2019 (COVID-19) can place an immense psychological strain on the infected patient. The psychological distress can linger after the initial recovery from the infection. Objective: This study aimed to evaluate the prevalence and predictors of provisional post-traumatic stress disorder (PTSD) in patients with cured COVID-2019. Methods: The baseline survey was conducted from 10 to 25 February 2020 in patients with COVID-19 in a designated hospital. Demographic and clinical characteristics were acquired, and...

Circular RNA circVRK1 suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating zinc finger protein ZNF652 expression via microRNA miR-337-3p

Cheng Cheng, Haoping Zhang, Zhipeng Dai & Jia Zheng
Circular RNA is an innovative kind of endogenous non-coding RNA, which could take part in tumorigenesis. Nonetheless, the potential molecular mechanisms of circVRK1 in the progression of osteosarcoma remain unresolved. In the current study, we initially investigated circVRK1 levels in osteosarcoma clinical samples and cell lines by qRT-PCR analysis and northern blot assay. RNase R treatments, RNA stability assay and nucleoplasmic separation assay were conducted to identify the characteristics of circVRK1. We adopted CCK-8, colony...

Chemical and Sooting Structures of Counterflow Diffusion Flames of Butanol Isomers: An Experimental and Modeling Study

Jizhou Zhang, Fuwu Yan, Peng Jiang, Mengxiang Zhou & Yu Wang
This work reports an experimental and numerical analysis on the sooting characteristics of butanol isomers. Light extinction and gas chromatography were used to measure soot and gas-phase species, respectively. Kinetic analysis of the tested flames was performed with detailed gas-phase mechanism and a sectional soot model. The present work aims to provide an understanding on the effects of butanol isomeric structures on sooting tendencies. For a comprehensive analysis, flames of both neat butanol fuels and...

Microbial profiling identifies potential key drivers in gastric cancer patients

Yonghong Li, Jia Wang, Mengge Wang, Yongshun Gao, Cheng-Yun Jin, Xiaojing Shi, Boyang Ji, Yongjun Wei & Hongmin Liu
Gastric cancer (GC) is the fifth most commonly diagnosed cancer and the third leading cause of cancer-related death in the world. Microbiota is believed to be associated with GC. Growing evidences showed Helicobacter pylori played a key role in GC development. However, little was known about the microbiota in gastric juices and tissues in GC patients, and thus it was difficult to understand other potential microbial causation for GC. Here, we collected the gastric juice...

Tumor-targeted Gd-doped mesoporous Fe3O4 nanoparticles for T1/T2 MR imaging guided synergistic cancer therapy

Shaohui Zheng, Shang Jin, Min Jiao, Wenjun Wang, Xiaoyu Zhou, Jie Xu, Yong Wang, Peipei Dou, Zhen Jin, Changyu Wu, Jingjing Li, Xinting Ge & Kai Xu
In this study, a novel intelligent nanoplatform to integrate multiple imaging and therapeutic functions for targeted cancer theranostics. The nanoplatform, DOX@Gd-MFe3O4 NPs, was constructed Gd-doped mesoporous Fe3O4 nanoparticles following with the doxorubicin (DOX) loading in the mesopores of the NPs. The DOX@Gd-MFe3O4 NPs exhibited good properties in colloidal dispersity, photothermal conversion, NIR triggered drug release, and high T1/T2 relaxicity rate (r1=9.64 mM−1s−1, r2= 177.71 mM−1s−1). Benefiting from the high MR contrast, DOX@Gd-MFe3O4 NPs enabled simultaneous...

Air-assisted liquid-liquid micro-extraction based on the solidification of a floating organic droplet for the determination of three strobilurin fungicides in water samples

Huihui Wang, Yue Wang, Xu Jing, Zhenjia Chen & Xiaowen Wang
A simple, fast, air-assisted liquid-liquid micro-extraction technique using the solidification of a floating organic droplet method was developed for the extraction of three strobilurin fungicides in water samples before analysis by high-performance liquid chromatography equipped with a diode array detector (HPLC-DAD). In this method, a low-density solvent (1-dodecanol) was used as the extraction solvent. Fine extraction solvent droplets were rapidly formed and dispersed into the water samples through air-pumping by syringe rather than an organic...

Air-assisted liquid-liquid micro-extraction based on the solidification of a floating organic droplet for the determination of three strobilurin fungicides in water samples

Huihui Wang, Yue Wang, Xu Jing, Zhenjia Chen & Xiaowen Wang
A simple, fast, air-assisted liquid-liquid micro-extraction technique using the solidification of a floating organic droplet method was developed for the extraction of three strobilurin fungicides in water samples before analysis by high-performance liquid chromatography equipped with a diode array detector (HPLC-DAD). In this method, a low-density solvent (1-dodecanol) was used as the extraction solvent. Fine extraction solvent droplets were rapidly formed and dispersed into the water samples through air-pumping by syringe rather than an organic...

Supplemental Material, sj-doc-1-gsj-10.1177_21925682211005732 - Clinicopathological and Prognostic Characteristics in Spinal Chondroblastomas: A Pooled Analysis of Individual Patient Data From a Single Institute and 27 Studies

Bo-Wen Zheng, Wei Huang, Fu-Sheng Liu, Tao-Lan Zhang, Xiao-Bin Wang, Jing Li, Guo-Hua Lv, Yi-Guo Yan & Ming-Xiang Zou
Supplemental Material, sj-doc-1-gsj-10.1177_21925682211005732 for Clinicopathological and Prognostic Characteristics in Spinal Chondroblastomas: A Pooled Analysis of Individual Patient Data From a Single Institute and 27 Studies by Bo-Wen Zheng, Wei Huang, Fu-Sheng Liu, Tao-Lan Zhang, Xiao-Bin Wang, Jing Li, Guo-Hua Lv, Yi-Guo Yan and Ming-Xiang Zou in Global Spine Journal

Upregulated HMGB1 levels in maternal–fetal interface of patients with unexplained recurrent spontaneous abortion from different sources

Jing Wang, Damin Zhu, Jiaqian Yin, Cong Ma, Xiaoqing Peng, Huijuan Zou, Yunxia Cao & Xiaofeng Xu
To investigate the expression and sources of high mobility group box 1 (HMGB1) protein in the maternal–fetal interface of patients with unexplained recurrent spontaneous abortion (URSA), and further to verify the role of HMGB1 in the etiology of URSA. 55 women at early pregnancy with URSA and 55 women undergoing selective termination of normal early pregnancy as control were included. The abortion tissues including villi and decidua were collected. The expression of HMGB1, CD45, CK7,...

MiR-3138 deteriorates the insulin resistance of HUVECs via KSR2/AMPK/GLUT4 signaling pathway

Yan Chen, Da Lin, Changxuan Shi, Liang Guo, Linhua Liu, Lin Chen, Ting Li, Ying Liu, Chengchao Zheng, Xintong Chi, Chun Meng & Yaoming Xue
Insulin resistance (IR) is a complex pathological condition resulting from the dysregulation of cellular response to insulin hormone in insulin-dependent cells and is recognized as a pathogenic hallmark and strong risk factor for metabolic syndrome. The present study aims to elucidate the molecular mechanism of the pathogenesis of IR. Here, we used human umbilical vein endothelial cells (HUVECs) to establish the IR cell model induced by 1 × 10−6 mmol/L insulin. After 48 h, reactive...

The safety and tolerability of alkaloids from Alstonia scholaris leaves in healthy Chinese volunteers: a single-centre, randomized, double-blind, placebo-controlled phase I clinical trial

Zhong-Ping Gou, Yun-Li Zhao, Lin-Ling Zou, Ying Wang, Shi-Qing Shu, Xiao-Hong Zhu, Li Zheng, Qi Shen, Zhu Luo, Jia Miao, Yong-Sheng Wang, Xiao-Dong Luo & Ping Feng
Capsule of alkaloids from the leaf of Alstonia scholaris (L.) R.Br. (Apocynaceae) (CALAS) is a new investigational botanical drug (No. 2011L01436) for bronchitis, post-infectious cough and asthma. To observe the clinical safety and tolerability of CALAS. Subjects were assigned to eight cohorts, and each received randomly CALAS or placebo in one of single ascending dose (SAD) of 8, 40, 120, 240, 360, 480, or in one of multiple ascending dose (MAD) of 40 or 120...

Two new terpenes from the aerial parts of Clematis chinensis Osbeck

Sun Ye, Liu Yan, Yan-Ping Sun, Xiao-Mao Li, Wang Si-Yi, Juan Pan, Wei Guan, Yuan Liu, Anam Naseem, Bing-You Yang & Hai-Xue Kuang
Two new acyclic sesquiterpenoids (1–2) and fourteen known monocyclic monoterpenoids (3–16) were isolated from the aerial parts of Clematis chinensis Osbeck. All compounds were isolated from C. chinensis for the first time. The structures of all compounds were characterized by spectroscopic methods (1 D, 2 D NMR and HRESIMS). In-vitro cytotoxic activity against two human cancer cell lines (MGC-803 and Ishikawa) of all the compounds were evaluated by CCK-8 assay.

Registration Year

  • 2021
    107

Resource Types

  • Text
    107

Affiliations

  • Zhengzhou University
    107
  • Shanghai Jiao Tong University
    80
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    79
  • Zhejiang University
    74
  • Capital Medical University
    74
  • Sichuan University
    73
  • Chinese Academy of Sciences
    65
  • Fudan University
    63
  • Huazhong University of Science and Technology
    57
  • Shandong University
    55