To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

Clear cell renal cell carcinoma, the most common type of renal cancer, is associated with poor survival. Ubiquitin-specific peptidase 2 regulates the molecular mechanisms of cancer cells. However, its mechanism in clear cell renal cell carcinoma remains unclear. Quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to assess ubiquitin-specific peptidase 2 expression in human clear cell renal cell carcinoma samples. Ubiquitin-specific peptidase 2 was weakly expressed in clear cell renal cell...

The short shoot phenomenon is an important phenotype underlying the economic value of roses – node number can directly affect shoot length. To elucidate the molecular mechanism of how day length affects shoot length, the effect of 8, 10, and 12 h day lengths on node number were studied. The number of nodes between the 8 and 12 h treatment groups significantly differed. Node development in the 12 h treatment group occurred significantly earlier than...

Organic contaminants of emerging concern (OCECs) originating from assorted sources have attracted increasing attention latterly as most of them are not under routine surveillance or effective regulation but possess persistent, bioaccumulative, and toxic potential. Massive terrestrial inputs and proximity to intense human activities have elevated OCEC contamination in many estuaries. The present review outlines the current knowledge on the environmental occurrence and fate, as well as bioavailability of nine classes of OCECs, i.e., per- and...

A pot experiment was performed to investigate the effect of phytoremediation (CK, using tall fescue), fungi remediation (GV, using Glomus versiforme), bacterial remediation (PS, using Pseudomonas fluorescens Ps2-6), and microbial-phytoremediation (GVPS, using three species) on removing polycyclic aromatic hydrocarbons (PAHs) and the microbial diversity in soils. Inoculation with G. versiforme and P. fluorescens could increase the biomass of tall fescue and the accumulation of phenanthrene (PHE) and pyrene (PYR) in plants. Among them, the highest...

Drought plays a prominent role in affecting ecosystem stability and ecosystem productivity. Based on eddy covariance and climatic observations during 2012–2020, the Fisher discriminant analysis method was employed to accurately detect drought occurrences. Furthermore, the ecosystem water sensitivity and its resistance to drought were quantified to evaluate the ecosystem stability. The results showed that the alpine meadow suffered drought most frequently at the beginning of the growing seasons. However, drought during the peak growing seasons...

Additional file 1: Supplementary table 1. Variables and coding used to construct the 30-item FI. Supplementary table 2. Baseline demographic characteristics and mortality by grades of frailty in the subjects aged over 65 years. Supplementary table 3. Comparison of FI-self-report vs FI-lab vs FI-combined for Prediction of 5-year mortality in the subjects aged over 65 years. Supplementary table 4. Baseline characteristics of the survived and dropped-out at 5 years. Supplementary fig 1. The flowchart of...

The urban development boundary (UDB) is a core issue in territorial spatial planning and remains a research focus in urban development. However, in the past, land use simulations of coastal cities have had some limitations. For example, new urban land outside the research boundary could not be effectively simulated, resulting in a large gap between the simulation results and reality. In this study, an artificial neural network (ANN) cellular automata (CA) model based on a...

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the...

Blueberries are rich in anthocyanins, which are plant-specific secondary metabolites that are a good source of plant nutrients. Although anthocyanins have been investigated, there is a lack of comprehensive detailed research that includes large numbers of blueberry cultivars, and the anthocyanin compositions of some cultivars are still unknown. Consequently, we determined the anthocyanin compositions of 62 cultivars using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS). In total, we identified 30 anthocyanins derived from five kinds of anthocyanidin....

Additional file 1. Original images for western blot.

Supplementary materials

Calmodulin-like interacting protein kinases play an important role in plant response to abiotic stresses and development. But the role of the CIPK gene in grapevine is unknown. In this study, VyCIPK1, isolated from the Chinese wild grape V. Yanshanesis, was strongly induced by salt stress. Overexpressing VyCIPK1 could induce AOC and AOS, and result in notably increased jamonate levels in tobacco. Under salt stress, transgenic plants showed higher germination rate, leaf number, and fresh weight...

In this work, hierarchical edge-rich Co3O4 nanosheets (e-Co3O4NSs) arrays were synthesised on the surface of Ni foam via a self-templating technique using metal-organic frameworks (MOFs) as precursors. The morphology and surface of Co3O4NSs could be tailed using the potassium hexacyanocobaltate solution. The synthesised e-Co3O4NSs shows that numerous small nanosheets were in-situ grown on the large nanosheet substrates, forming a core-shell structure, which could provide more active sites for electrochemical reactions. Benefiting from the structural superiorities,...

In this work, we have successfully fabricated Sr-doped porous LaFeO3 samples via sol–gel method. The results reveal that Sr2+ cation is effectively doped into LaFeO3 crystal lattice substituting La3+ cation. The visible light catalytic performance of the materials was evaluated by the degradation of 2,4-dichlorophenol (2,4-DCP) and Rhodamine B (RhB). The amount-optimized Sr-doped porous LaFeO3 sample exhibited outstanding visible-light catalytic performance for the degradation of the model pollutants compared to the porous LaFeO3 alone. The...

Additional file 2: Figure S1. Original blot and images. Figure S2. HMGB2 expression is not correlated with survival of patients with LUSC.

White clover (Trifolium repens L.) is an important legume forage, which is widely distributed in cool-season regions. Therefore, cold stress is a major environmental factor limiting its growth and production, while little is known about the its cold tolerance at molecular level. In the present study, we performed time-course RNA-seq analysis under cold stress. RNA-seq results suggested that genes associated with “oxidoreductase activity” and “transcription regulator activity” are more likely related to the response of...

Additional file 1: Figure S1. Associations between L-carnitine and the risk of gestational diabetes mellitus (GDM) in Chinese women.

Additional file 1: Figure S1. DLS analysis of F68@TA prepared by different mass ratio of F68 and TA. (n=3). Figure S2. TEM images of F68@TA@Ag NPs prepared with different content of AgNO3. Figure S3. (A) Size distributions and (B) zeta potentials of F68@TA, F68@TA/CDDP, and AINR solutions. Figure S4. Tyndall Effects of F68@TA/CDDP solution at different pHs for 1 h. Figure S5. DLS analysis of F68@TA/CDDP treated with PBS at different pHs for 48 h....

Microplastics (MPs) have a tremendous potential to sorb hydrophobic organic contaminants in the soil environment. The effects of MPs in the soil systems, however, remain largely unexplored. The objective of this study was to investigate the impact of polyethylene and polystyrene MPs on the transport of the benzo[a]anthracene (BaA) and benzo[a]pyrene (BaP) in soil matrices. In this study, we investigated the sorption process of BaA and BaP in batch sorption experiments in a laboratory and...

Supplementary Material 1

Additional file 2: Clinical stage-specific disability weights and distribution of breast cancer in females in China.

Additional file 4: Age-specific number of females and standardized life expectancy

Additional file 3:

Additional file 2.