1,142 Works

Implementation of novel boolean logic gates for IMPLICATION and XOR functions using riboregulators

Chaoxin Chen, Qi Wu, Qingying Ke, Ting Wang, Yifan Zhang, Feiwen Wei, Xiaolong Wang & Guanglei Liu
To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

Ubiquitin-specific peptidase 2 inhibits epithelial-mesenchymal transition in clear cell renal cell carcinoma metastasis by downregulating the NF-κB pathway

Jiachen Duan, Mengyuan Jin, Dongjing Yang, Jihua Shi, Jie Gao, Danfeng Guo, Hongwei Tang, Shuijun Zhang & Baoping Qiao
Clear cell renal cell carcinoma, the most common type of renal cancer, is associated with poor survival. Ubiquitin-specific peptidase 2 regulates the molecular mechanisms of cancer cells. However, its mechanism in clear cell renal cell carcinoma remains unclear. Quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to assess ubiquitin-specific peptidase 2 expression in human clear cell renal cell carcinoma samples. Ubiquitin-specific peptidase 2 was weakly expressed in clear cell renal cell...

Molecular evidence to the day length in regulating the short shoot phenomenon during summer in roses (Rosa sp.)

Li Wang, Wan Jiang, Wuhua Zhang, Weitong Chen, Yu Mo, Tong Zhou, Jinzhu Zhang & Daidi Che
The short shoot phenomenon is an important phenotype underlying the economic value of roses – node number can directly affect shoot length. To elucidate the molecular mechanism of how day length affects shoot length, the effect of 8, 10, and 12 h day lengths on node number were studied. The number of nodes between the 8 and 12 h treatment groups significantly differed. Node development in the 12 h treatment group occurred significantly earlier than...

Organic contaminants of emerging concern in global estuaries: Environmental occurrence, fate, and bioavailability

Yan Wu, Ruihe Jin, Qiqing Chen, Xinyu Du, Jing Yang & Min Liu
Organic contaminants of emerging concern (OCECs) originating from assorted sources have attracted increasing attention latterly as most of them are not under routine surveillance or effective regulation but possess persistent, bioaccumulative, and toxic potential. Massive terrestrial inputs and proximity to intense human activities have elevated OCEC contamination in many estuaries. The present review outlines the current knowledge on the environmental occurrence and fate, as well as bioavailability of nine classes of OCECs, i.e., per- and...

Effect of arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) on microorganism of phenanthrene and pyrene contaminated soils

Wei Li, Wen-bin Li, Li-jun Xing & Shao-xia Guo
A pot experiment was performed to investigate the effect of phytoremediation (CK, using tall fescue), fungi remediation (GV, using Glomus versiforme), bacterial remediation (PS, using Pseudomonas fluorescens Ps2-6), and microbial-phytoremediation (GVPS, using three species) on removing polycyclic aromatic hydrocarbons (PAHs) and the microbial diversity in soils. Inoculation with G. versiforme and P. fluorescens could increase the biomass of tall fescue and the accumulation of phenanthrene (PHE) and pyrene (PYR) in plants. Among them, the highest...

Influences of drought on the stability of an alpine meadow ecosystem

Tao Zhang, Ximeng Ji, Mingjie Xu, Guang Zhao, Zhoutao Zheng, Yuanyuan Tang, Ning Chen, Juntao Zhu, Yongtao He & Yangjian Zhang
Drought plays a prominent role in affecting ecosystem stability and ecosystem productivity. Based on eddy covariance and climatic observations during 2012–2020, the Fisher discriminant analysis method was employed to accurately detect drought occurrences. Furthermore, the ecosystem water sensitivity and its resistance to drought were quantified to evaluate the ecosystem stability. The results showed that the alpine meadow suffered drought most frequently at the beginning of the growing seasons. However, drought during the peak growing seasons...

Additional file 1 of A frailty index based on routine laboratory data predicts increased risk of mortality in Chinese community-dwelling adults aged over 55 years: a five-year prospective study

Chunxiu Wang, Xianghua Fang, Zhe Tang, Yang Hua, Zhongying Zhang, Xiang Gu, Beibei Liu & Xunming Ji
Additional file 1: Supplementary table 1. Variables and coding used to construct the 30-item FI. Supplementary table 2. Baseline demographic characteristics and mortality by grades of frailty in the subjects aged over 65 years. Supplementary table 3. Comparison of FI-self-report vs FI-lab vs FI-combined for Prediction of 5-year mortality in the subjects aged over 65 years. Supplementary table 4. Baseline characteristics of the survived and dropped-out at 5 years. Supplementary fig 1. The flowchart of...

Offshore Island Connection Line: A new perspective of coastal urban development boundary simulation and multi-scenario prediction

Fei He, Jun Yang, Yuqing Zhang, Dongqi Sun, Li Wang, Xiangming Xiao & Jianhong Xia
The urban development boundary (UDB) is a core issue in territorial spatial planning and remains a research focus in urban development. However, in the past, land use simulations of coastal cities have had some limitations. For example, new urban land outside the research boundary could not be effectively simulated, resulting in a large gap between the simulation results and reality. In this study, an artificial neural network (ANN) cellular automata (CA) model based on a...

Use of sponge iron dosing in baffled subsurface-flow constructed wetlands for treatment of wastewater treatment plant effluents during autumn and winter

Muyu Feng, Jinming Liang, Peng Wang, Ya’e Wang & Jie Li
Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the...

Identification and quantification of anthocyanins of 62 blueberry cultivars via UPLC-MS

Silu Wang, Bowei Wang, Kun Dong, Jing Li, Yadong Li & Haiyue Sun
Blueberries are rich in anthocyanins, which are plant-specific secondary metabolites that are a good source of plant nutrients. Although anthocyanins have been investigated, there is a lack of comprehensive detailed research that includes large numbers of blueberry cultivars, and the anthocyanin compositions of some cultivars are still unknown. Consequently, we determined the anthocyanin compositions of 62 cultivars using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS). In total, we identified 30 anthocyanins derived from five kinds of anthocyanidin....

Additional file 1 of 5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo

Sushan Yang, Linxin Wang, Xiping Pan, Yueyun Liang, Yuehan Zhang, Jing Li & Beixian Zhou
Additional file 1. Original images for western blot.

Supplementary document for Optical Microscopy with Large Tilt Angle and Long Focus Length for a Nano-size Angle Resolved Photoemission Spectroscopy - 6062104.pdf

Chenyang Yue, Hong Jiang, Chuan Guo, Tianzhi Li, Siyan Yao, Shuo Zhang, Dan Zhang, Shenyue Zeng, Meixiao Wang & Xiaojun Xu
Supplementary materials

Ectopic expression of VyCIPK1 gene, isolated from wild grape Vitis yanshanesis J, X. Chen., confers the tolerance to salt in transgenic tobacco

Ziguo Zhu, Lingmin Dai, Guangxia Chen, Guanghui Yu, Xiujie Li, Zhen Han & Bo Li
Calmodulin-like interacting protein kinases play an important role in plant response to abiotic stresses and development. But the role of the CIPK gene in grapevine is unknown. In this study, VyCIPK1, isolated from the Chinese wild grape V. Yanshanesis, was strongly induced by salt stress. Overexpressing VyCIPK1 could induce AOC and AOS, and result in notably increased jamonate levels in tobacco. Under salt stress, transgenic plants showed higher germination rate, leaf number, and fresh weight...

Surface engineering of MOFs-derived Co3O4 nanosheets for high-performance supercapacitor

Xuemin Yin, Huimin Liu, Kezhi Li & Jinhua Lu
In this work, hierarchical edge-rich Co3O4 nanosheets (e-Co3O4NSs) arrays were synthesised on the surface of Ni foam via a self-templating technique using metal-organic frameworks (MOFs) as precursors. The morphology and surface of Co3O4NSs could be tailed using the potassium hexacyanocobaltate solution. The synthesised e-Co3O4NSs shows that numerous small nanosheets were in-situ grown on the large nanosheet substrates, forming a core-shell structure, which could provide more active sites for electrochemical reactions. Benefiting from the structural superiorities,...

High-performance visible-light active Sr-doped porous LaFeO3 semiconductor prepared via sol–gel method

Xi-Tao Yin, Hua Huang, Jie-Li Xie, Davoud Dastan, Jing Li, Ying Liu, Xiao-Ming Tan, Xiao-Chun Gao, Wahab Ali Shah & Xiao-Guang Ma
In this work, we have successfully fabricated Sr-doped porous LaFeO3 samples via sol–gel method. The results reveal that Sr2+ cation is effectively doped into LaFeO3 crystal lattice substituting La3+ cation. The visible light catalytic performance of the materials was evaluated by the degradation of 2,4-dichlorophenol (2,4-DCP) and Rhodamine B (RhB). The amount-optimized Sr-doped porous LaFeO3 sample exhibited outstanding visible-light catalytic performance for the degradation of the model pollutants compared to the porous LaFeO3 alone. The...

Additional file 2 of Identification of HMGB2 associated with proliferation, invasion and prognosis in lung adenocarcinoma via weighted gene co-expression network analysis

Xie Qiu, Wei Liu, Yifan Zheng, Kai Zeng, Hao Wang, Haijun Sun & Jianhua Dai
Additional file 2: Figure S1. Original blot and images. Figure S2. HMGB2 expression is not correlated with survival of patients with LUSC.

Time-course RNA-seq analysis provides an improved understanding of genetic regulation in response to cold stress from white clover (Trifolium repens L.)

Xueqi Zhang, Huanhuan Yang, Manman Li, Chao Chen, Yan Bai, Donglin Guo, Changhong Guo & Yongjun Shu
White clover (Trifolium repens L.) is an important legume forage, which is widely distributed in cool-season regions. Therefore, cold stress is a major environmental factor limiting its growth and production, while little is known about the its cold tolerance at molecular level. In the present study, we performed time-course RNA-seq analysis under cold stress. RNA-seq results suggested that genes associated with “oxidoreductase activity” and “transcription regulator activity” are more likely related to the response of...

Additional file 1 of Interactions of CDKAL1 rs7747752 polymorphism and serum levels of L-carnitine and choline are related to increased risk of gestational diabetes mellitus

Hui Wang, Jing Li, Jinnan Liu, Junhong Leng, Weiqin Li, Zhijie Yu, Claudia H. T. Tam, Gang Hu, Ronald C. W. Ma, Zhongze Fang, Ying Wang & Xilin Yang
Additional file 1: Figure S1. Associations between L-carnitine and the risk of gestational diabetes mellitus (GDM) in Chinese women.

Additional file 1 of Boosting cisplatin chemotherapy by nanomotor-enhanced tumor penetration and DNA adducts formation

Lihua Xu, Kaixiang Zhang, Xing Ma, Yingying Li, Yajie Jin, Chenglin Liang, Yong Wang, Wendi Duan, Hongling Zhang, Zhenzhong Zhang, Jinjin Shi, Junjie Liu, Yunlong Wang & Wentao Li
Additional file 1: Figure S1. DLS analysis of F68@TA prepared by different mass ratio of F68 and TA. (n=3). Figure S2. TEM images of F68@TA@Ag NPs prepared with different content of AgNO3. Figure S3. (A) Size distributions and (B) zeta potentials of F68@TA, F68@TA/CDDP, and AINR solutions. Figure S4. Tyndall Effects of F68@TA/CDDP solution at different pHs for 1 h. Figure S5. DLS analysis of F68@TA/CDDP treated with PBS at different pHs for 48 h....

Adsorption behaviour and mechanism of polycyclic aromatic hydrocarbons onto typical microplastics in a soil solution

Zhen Li, Lina Sun & Hui Wang
Microplastics (MPs) have a tremendous potential to sorb hydrophobic organic contaminants in the soil environment. The effects of MPs in the soil systems, however, remain largely unexplored. The objective of this study was to investigate the impact of polyethylene and polystyrene MPs on the transport of the benzo[a]anthracene (BaA) and benzo[a]pyrene (BaP) in soil matrices. In this study, we investigated the sorption process of BaA and BaP in batch sorption experiments in a laboratory and...

Additional file 1 of Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonist

Yu Bai, Xin Zhang, Jie Zheng, Ziyi Liu, Zhenfan Yang & Xiaolin Zhang
Supplementary Material 1

Additional file 2 of Estimating disability-adjusted life years for breast cancer and the impact of screening in female populations in China, 2015–2030: an exploratory prevalence-based analysis applying local weights

Xin-Xin Yan, Juan Zhu, Yan-Jie Li, Meng-Di Cao, Xin Wang, Hong Wang, Cheng-Cheng Liu, Jing Wang, Yang Li & Ju-Fang Shi
Additional file 2: Clinical stage-specific disability weights and distribution of breast cancer in females in China.

Additional file 4 of Estimating disability-adjusted life years for breast cancer and the impact of screening in female populations in China, 2015–2030: an exploratory prevalence-based analysis applying local weights

Xin-Xin Yan, Juan Zhu, Yan-Jie Li, Meng-Di Cao, Xin Wang, Hong Wang, Cheng-Cheng Liu, Jing Wang, Yang Li & Ju-Fang Shi
Additional file 4: Age-specific number of females and standardized life expectancy

Additional file 3 of Using a mixed method to identify communication skills training priorities for Chinese general practitioners in diabetes care

Mi Yao, Gang Yuan, Kai Lin, Lijuan Liu, Hao Tang, Jieying Xie, Xinxin Ji, Rongxin Wang, Binkai Li, Jiajia Hao, Huichang Qiu, Dongying Zhang, Hai Li, Shamil Haroon, Dawn Jackson, Wei Chen, Kar Keung Cheng & Richard Lehman
Additional file 3:

Additional file 2 of Gastric cancer-derived exosomal miR-519a-3p promotes liver metastasis by inducing intrahepatic M2-like macrophage-mediated angiogenesis

Shengkui Qiu, Li Xie, Chen Lu, Chao Gu, Yiwen Xia, Jialun Lv, Zhe Xuan, Lang Fang, Jing Yang, Lu Zhang, Zheng Li, Weizhi Wang, Hao Xu, Bowen Li & Zekuan Xu
Additional file 2.

Registration Year

  • 2022

Resource Types

  • Text


  • Zhengzhou University
  • Shanghai Jiao Tong University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Zhejiang University
  • Capital Medical University
  • Sichuan University
  • Chinese Academy of Sciences
  • Nanjing Medical University
  • Fudan University
  • Peking University