DNA topoisomerase II alpha (TOP2A) reportedly plays a crucial role in several cancers, however, the precise regulatory role of TOP2A in metastatic characteristics of glioma is still poorly understood. Herein, we sought to elucidate the mechanisms by which TOP2A affects the metastatic phenotypes of glioma. We observed that a high level of TOP2A expression was dramatically linked with inferior survival in glioma patients while silencing of TOP2A impaired glioma cell proliferation and aggressiveness. TOP2A was...

DNA topoisomerase II alpha (TOP2A) reportedly plays a crucial role in several cancers, however, the precise regulatory role of TOP2A in metastatic characteristics of glioma is still poorly understood. Herein, we sought to elucidate the mechanisms by which TOP2A affects the metastatic phenotypes of glioma. We observed that a high level of TOP2A expression was dramatically linked with inferior survival in glioma patients while silencing of TOP2A impaired glioma cell proliferation and aggressiveness. TOP2A was...

Species interactions alter with environmental change, which influences the relationship between biodiversity and ecosystem functioning (BEF). How BEF relationships change with environments requires knowledge of the changes of functional attributes in individual species in response to environmental change. Here, we assembled experimental plant communities with different species richness (1, 2, 4, 8, and 16) and exposed them to a drought stress gradient (control, moderate and intensive drought stress). Functional redundancy decreased with increasing drought stress...

Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Pot and field trials were conducted to investigate Cd uptake and phytoremediation efficiency of two Brassica napus cultivars (QY-1 and SYH) with applied water-soluble chitosan (WSC, Pot: 0, 2% and 4%; Field: 0 and 10 g·m−2) grown in Cd-contaminated soils. The results from the pot and field trials generally showed that WSC treatments significantly increased Cd concentrations in shoot and root tissues by 33.77–159.71% (except for SYH/JY) and 7.42–168.71% of two B. napus cultivars compared...

Increase in air pollution caused by acidic gases, such as nitrogen dioxide, call for the need to study potential effects on the structure of textile materials. Wool is a natural protein fiber which is easily damaged due to rich reaction positions and cross-linked bonds in the fiber. This study reports, for the first time, effect of nitrogen dioxide, as a model acid gas pollutant, on the structure of wool. On the macro level, wool fabric...

Lotus rhizome starch (LRS) is an important traditional processed product of lotus rhizome in China due to its good taste and high nutritional value. However, adulterated lotus rhizome starches are prevalent on the market. In this study, a novel method for the rapid detection of adulteration in LRS based on color reaction between ferrous sulfate (FS) and (-)-gallocatechin (GC) in LRS was studied. The polyphenols in LRS were analyzed by liquid chromatography-mass spectrometry (LC-MS/MS) as...

There are few studies on denitrification related to the nitrogen cycle in Ebinur Lake wetland. This study aimed to explore the response of the diversity and composition of denitrifying bacteria to the environmental factors in wetland, so as to obtain more information about the community structure of denitrifying bacteria driven by environmental factors. Using the genes encoding nitrite reductase (nirS and nirK) as molecular markers, we analysed the seasonal changes in the diversity of denitrifying...

Additional file 2: Supplemental Table 2. The kappa coefficients of categorical variables between three radiologists.

It has been discovered that many plants accumulate proline in response to osmotic stress. However, the interaction between salinity stress and proline metabolism-related genes has not been unequivocally identified in Spartina alterniflora. In our research, we successfully cloned key genes: SaP5CS1 and SaP5CS2, SaOAT, SaProT and correlated with expression profiles including SaPDH were also investigated. As the key enzyme genes for proline metabolism, the up-regulated expression of SaP5CS2 played a leading part under salt stress....

Additional file 2.

Additional file 8.

Additional file 2.

Researchers have investigated miR-130b-3p in lung disease pathology, such as lung fibrosis. The present study was performed to elucidate the miR-130b-3p-involved mechanism in acute lung injury (ALI) through delivery by mesenchymal stem cells-derived exosomes (MSCs-Exo). ALI mouse models were induced via intratracheal administration of lipopolysaccharide (LPS) and treated with MSCs-Exo. Lung dry-wet (W/D) ratio, inflammatory factors in the bronchoalveolar lavage fluid, pathological damage and apoptosis in the lung tissues were analyzed. Expression levels of miR-130b-3p...

Supplementary Material containing S1, S2 and S3.

Additional file 1: Supplement Table 1. Baseline comparison of patients and healthy donors.

Additional file 2: Supplement Table 2. Events happened in IST-R and IST-NR group.

Supplementary Material 1

Supplementary Material 2

Nitric oxide (NO) is involved not only in the regulation of plant growth, development, and stress responses but also in the regulation of plant-microbe interactions. Here, we demonstrate that Piriformospora indica can induce tobacco nitrate reductase to produce a NO signal in roots which enhances nitrogen uptake capacity by inducing the expression of ammonium and nitrate transporter genes and the development of lateral root and root hair, thereby promoting tobacco growth. In addition, the NO...

Supplementary

figures of the result of the rest animals and humans

The benefits of potent antithrombotic therapy usually come at the expense of a higher risk of bleeding. The efficacy and safety of ticagrelor in elderly East Asian populations remains debated due to the concerns about the imbalance of ischemic and bleeding risks. This study aimed to compare the impact of clopidogrel with ticagrelor on clinical outcomes in East Asian patients aged ≥75 years with acute coronary syndrome (ACS) using data from an institutional registry. We...

Osteoporosis is a worldwide disease that seriously affects the quality of life and survival rate of the elderly. The detection of bone biomarkers will provide supplementary information on bone mineral density, contributing to the accurate diagnosis of osteoporosis and better health care for prevention. This study aimed to investigate the efficacy of oxidative stress markers—8-oxo-7,8-dihydroguanine (8-oxoGsn) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGsn) in the assessment of osteoporosis. We conducted a cross-sectional study among menopausal women with a mean...