Background: Novel coronavirus pneumonia (COVID-19) is prevalent around the world. We aimed to describe epidemiological features and clinical course in Shanghai. Methods: We retrospectively analysed 325 cases admitted at Shanghai Public Health Clinical Center, between January 20 and February 29, 2020. Results: 47.4% (154/325) had visited Wuhan within 2 weeks of illness onset. 57.2% occurred in 67 clusters; 40% were situated within 53 family clusters. 83.7% developed fever during the disease course. Median times from...

An enclosed air-lift photobioreactor (ALPBR) is considered an efficient lab-scale bioreactor for microalgae cell growth and lipid biosynthesis. However, fluid-induced shear stress and mixing are two main factors that affect physiological metabolism in microalgal cell cultures. Herein, a 50-L ALPBR after being designed and manufactured was evaluated for microalgal suspension culture. Moreover, computational fluid dynamics (CFD) simulation was used to characterize the hydrodynamics of ALPBR. Specifically, two model microalgae species Chlorella vulgaris and Chlorella protothecoides...

Additional file 1. The search strategy example of PubMed.

COVID-19 is caused by SARS-CoV-2 infection and was initially discovered in Wuhan. This outbreak quickly spread all over China and then to more than 20 other countries. SARS-CoV-2 fluorescent microsphere immunochromatographic test strips were prepared by the combination of time-resolved fluorescence immunoassay with a lateral flow assay. The analytical performance and clinical evaluation of this testing method was done and the clinical significance of the testing method was verified. The LLOD of SARS-CoV-2 antibody IgG...

Epidermal growth factor receptor (EGFR) is a promising target for the treatment of different types of malignant tumors. Therefore, a combined molecular modeling study was performed on a series of quinazoline derivatives as EGFR inhibitors. The optimum ligand-based CoMFA and CoMSIA models showed reliable and satisfactory predictability (with R2cv=0.681, R2ncv=0.844, R2pred=0.8702 and R2cv=0.643, R2ncv=0.874, R2pred=0.6423). The derived contour maps provide structural features to improve inhibitory activity. Furthermore, the contour maps, molecular docking, and molecular dynamics...

This work studied the physical properties of three deep eutectic solvents (DESs) and their catalytic activity in the esterification of palmitic acid and ethanol. Three types of deep eutectic solvents (DESs) were successfully prepared using p-toluene sulfonic acid monohydrate (PTSA) as hydrogen bond donor (HBD) with dodecyl trimethyl ammonium chloride (DTAC), tetradecyl trimethyl ammonium chloride (TTAC) and octadecyl trimethyl ammonium chloride (OTAC) as hydrogen bond acceptor (HBA). The freezing points of DESs were measured in...

Additional file 2: Supplemental Table 2. The kappa coefficients of categorical variables between three radiologists.

Some individuals with acute pancreatitis (AP) suffer from pancreatic necrosis. Diabetes affects the severity of AP, but whether diabetes influences pancreatic necrosis is unclear. This study aims to investigate the clinical characteristics of AP patients with and without diabetes as well as analyze the risk factors of pancreatic necrosis. A total of 625 AP patients participated in the study. Clinical and laboratory data were retrieved. Multivariate logistic regression analysis was used to identify the risk...

The demonstration of batch-to-batch consistency is indispensable for quality control of vaccines. We conducted a randomized, double-blind, parallel-controlled trial to evaluate the immunogenicity consistency of a single shot of Ad5-nCoV in healthy adults who had not previously received any COVID-19 vaccine. All eligible participants were randomly assigned equally to receive one of the three consecutive batches of Ad5-nCoV (5 × 1010 viral particles/vial, 0.5 mL). The primary endpoint was geometric mean titers (GMTs) of serum...

The reduction of CO2 into valuable chemicals through electrocatalytic techniques provides a potential strategy to alleviate energy crises and environmental pollution. In this study, 2D M3(THT)2 (M = Fe, Co, Ni, Ru, Rh, Pd, THT = 2,3,6,7,10,11-triphenylenehexathiolate) framework as CO2 reduction reaction (CO2RR) electrocatalysts was investigated by using the density functional method. The results showed that the studied catalysts are stable both thermodynamically and electrochemically. Rh3(THT)2 exhibits the best catalytic performance to produce CH4 with...

Supplementary Material containing S1, S2 and S3.

RAS protein activator like 2 (Rasal2) exerts pro-proliferative effect in several types of cells. However, whether Rasal2 is involved in the regulation of pulmonary artery smooth muscle cell (PASMC) remains unclear. In the current study, we explored the role of Rasal2 in proliferation and migration of PASMC during the development of pulmonary arterial hypertension (PAH). We found that the protein level of Rasal2 was increased in both pulmonary arteries of chronic hypoxia-induced pulmonary hypertension (CH-PH)...

In this study, 18 novel thiochroman-4-one derivatives incorporating oxime ether and 1,3,4-oxadiazole thioether moieties were synthesized and their structures analyzed by 1H NMR, 13C NMR, and HRMS. Their in vitro antibacterial activities against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac) as well as antifungal activities against Botrytis cinerea (B. cinerea), Verticillium dahliae (V. dahliae), and Fusarium oxysporum (F. oxysporum) were determined. Bioassay results showed that compound 6-chloro-2-(5-(methylthio)-1,3,4-oxadiazol-2-yl)thiochroman-4-one O-methyl oxime (7a) was...

In this study, 18 novel thiochroman-4-one derivatives incorporating oxime ether and 1,3,4-oxadiazole thioether moieties were synthesized and their structures analyzed by 1H NMR, 13C NMR, and HRMS. Their in vitro antibacterial activities against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac) as well as antifungal activities against Botrytis cinerea (B. cinerea), Verticillium dahliae (V. dahliae), and Fusarium oxysporum (F. oxysporum) were determined. Bioassay results showed that compound 6-chloro-2-(5-(methylthio)-1,3,4-oxadiazol-2-yl)thiochroman-4-one O-methyl oxime (7a) was...

Additional file 1. Supplementary Material for a Successful Hybrid Deep Learning Model aiming at Promoter Identification.

Lamivudine is a first-line medication used for human immunodeficiency virus (HIV) treatment. To date, the population pharmacokinetics of lamivudine in Chinese HIV-infected adults have not been assessed. This study aimed to develop a population pharmacokinetic model for oral lamivudine in Chinese HIV-infected adults and to determine the optimal lamivudine dosage regimens. A total of 1113 samples, from 828 Chinese HIV-infected patients treated with lamivudine 300 mg every 24 hours, were pooled from two open-label, prospective...

Supplemental material, sj-pdf-1-imr-10.1177_03000605221132711 for New onset atrial fibrillation during orthotopic liver transplantation induced by iced saline injection for transpulmonary thermodilution: a case report by Xin Li, Xu Wang and Zheng Guan in Journal of International Medical Research

Additional file 1: Table S1. Primers used for PCR analysis.

Additional file 1: Fig. S1. Fragment recruitment analysis. Fig. S2. The relative abundance of transporter proteins in metaproteome. Fig. S3. Profiles of transporter genes in the assembled MAGs within Epsilonbacteraeota and Gammaproteobacteria.

Additional file 1. Database Search Strategy in English.

Supplementary Material 3

Additional file 3: Table S3. Comparison of clinical outcomes between early and delayed initiation of rituximab subgroups in rituximab cohort

Additional file 1: Figure S1 Characterization of ZON. A, B ZON was synthesized and the actual size and surface charge were observed by TEM and zeta potential analyzer. Figure S2 Effect of ZON on HcerEpic cells. A HcerEpic cells were treated with ZON. The cell growth was analyzed by colony formation assays. Ns no significant. Figure S3 ZON inhibits cell growth of cervical cancer in vivo by targeting CD164. A–D Tumor growth of Hela cell...

Supplementary Table

Additional file 2: Fig. S2. The predictivity of 12 DE-MRGs model for other cancers. A The univariate cox analysis showed that the risk score, based on our 12 DE-MRGs, significantly associated with other 12 types of TCGA cancers. B Kaplan–Meier curves for OS in the other 12 types of TCGA cancers. The patients were divided in to high- and low-risk groups based on the median value of risk score.