A QuEChERS method was developed to simultaneously quantify isoprothiolane and tebuconazole in paddy field samples by using UPLC-MS/MS. The method has been successfully applied in determining isoprothiolane and tebuconazole in brown rice, polished rice, rice husk and straw. The dissipation behaviour of isoprothiolane and tebuconazole in rice followed first-order kinetics, with a linear correlation coefficient of 0.8283 to 0.9877. The degradation half-life of isoprothiolane in rice grain was 6.5–25.7 days, whereas that of tebuconazole was...

Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Heavy metal ion pollution has become a serious problem. In this paper, a new type of adsorbent, reduced graphene oxide grafted by 4-sulfophenylazo groups (RGOS), was synthesized to adsorb heavy metal ions in an aqueous solution via two kinds of adsorption modes, ion exchange and coordination. The maximum adsorption capacities of the RGOS for Pb(II), Cu(II), Ni(II), Cd(II) and Cr(III) were 689, 59, 66, 267 and 191 mg/g, respectively. Adsorption equilibrium time of RGOS for...

Curcumin (Cur) is a flavonoid derived from Curcuma longa L. that has been shown to have a variety of biological activities, but some previous studies have described its non-negligible negative effects on female reproduction and embryo development. To further explore the toxic stress effect, this study investigated apoptosis and autophagy of healthy buffalo (Bubalus bubalis) derived granulosa cells (GCs) exposed to Cur and/or autophagy inhibitors. Results showed that Cur declined viability of GCs in a...

Two new abietane diterpenoids, phlecarinatone A (1) and phlecarinatone B (2), along with two known analogues (3 and 4), were isolated from Phlegmariurus carinatus. The structures of 1 − 4 were unambiguously elucidated by comprehensive spectroscopic analyses. Abietane diterpenoids were isolated from the plant for the first time. All isolates were tested for their neuroprotective activities against H2O2-induced SH-SY5Y cells injury, and compound 2 showed moderate effect at the concentrations ranging from 5 ∼ 20...

Additional file 1: Fig. S1. Plasma cortisol concentrations in response to ACTH-challenge of postpartum dairy cows supplemented with n-3 (21 DIM). Fig. S2. Distribution of adipocytes according to adipocyte area of adipose tissue of PP dairy cows supplemented with n-3 fatty acids or control diet. Fig. S3. Peripheral blood mononuclear cells (PBMC) protein abundance of ECS-related proteins during the 1st week PP of dairy cows supplemented with n-3 fatty acids. Fig. S4. Western blot images...

Pregnant women’s influenza vaccination uptake was low, although being recommended as a priority. We conducted a cross-sectional questionnaire survey in China from June to July 2021. Hierarchical regression analysis and structural equation modeling were performed based on the extended theory of planned behavior, in which attitudes, subjective norms, perceived behavioral control, and intention each affect vaccine hesitancy; response efficacy, knowledge, vulnerability, and severity were added as extended dimensions; vaccination history played as a moderator. Totally,...

Additional file 1: Fig. S1. Jitter and hist plots (statin plus evolocumab-based PSM).

Post-orthopaedic operative pain is a serious concern that often requires the administration of analgesics; however, the optimal time of analgesic administration is still inconclusive. Perioperative analgesia is administrating pre-emptive analgesia before and during the surgery followed by postoperative analgesia to decrease the procedure associated nociceptive response. We aim to assess perioperative meloxicam versus postoperative meloxicam for pain control after orthopaedic operations. A systematic review and meta-analysis involving randomized controlled trials from PubMed, Embase, Scopus, WOS,...

Additional file 4: Supplementary Figures S1. Enriched target class of TOP (top) and HDAC (bottom) in the predictions. Supplementary Figures S2. Cell viability assay valuation of MMF. Supplementary Figures S3. RNAseq analysis of MMF-treated SF8628 (A) and SU-DIPG- IV (B) cells. Supplementary Figures S4. Expression of IMPDH2 in pediatric high-grade glioma cells. Supplementary Figures S5. In vivo anti-tumor activity of MMF by CED in orthotopic patient-derived DIPG xenografts.

Improving the cell selectivity of anticancer peptides (ACPs) is a major hurdle in their clinical utilisation. In this study, a new acid-activated ACP was designed by conjugating a cationic ACP LK to its anionic binding partner peptide (LEH) via a disulphide linker to trigger antitumor activity at acidic pH while masking its killing activity at normal pH. Three anionic binding peptides containing different numbers of glutamic acid (Glu) and histidine were engineered to obtain an...

Additional file 1.

Additional file 4. Markdown file of example data analysis.

Additional file 1: Figure S1. (A) Confocal imaging of SLAMF7 and Iba1 on mouse brain tissue during peak EAE. Magnifications 1 and 2 highlight regions with SLAMF7+ Iba1− non-microglial cells and SLAMF7− Iba1+ microglia, respectively. (B–E) Notable co-expression patterns of SLAMF7 with other regulatory markers on various CNS immune cell subsets during EAE. (B) Co-expression of SLAMF7 and PD-1 on CD8+ T cells, (C) SLAMF7 co-expression with CD38 on CD8+ T cells, (D) co-expression of...

Additional file 1. Supplemental methods and data: biomass composition, hydrolysate composition, and statistical analyses.

Additional file 1: Figure S1. More representative images of blastocysts from frozen-thawed D5 (A) and frozen-thawed D6 (B). Figure S2. Representative images of blastocysts from frozen-thawed D5 (A) and frozen-thawed D6 (B) during aspiration. Figure S3. Comparisons of the pregnancy ratio (A) and abortion ratio (B) between patients received high quality of blastocyst transplantation from frozen-thawed D5 and general quality from frozen-thawed D6. Fisher’s exact test. Figure S4. Comparisons of the pregnancy ratio (A) and...

Additional file 1: Figure S1. More representative images of blastocysts from frozen-thawed D5 (A) and frozen-thawed D6 (B). Figure S2. Representative images of blastocysts from frozen-thawed D5 (A) and frozen-thawed D6 (B) during aspiration. Figure S3. Comparisons of the pregnancy ratio (A) and abortion ratio (B) between patients received high quality of blastocyst transplantation from frozen-thawed D5 and general quality from frozen-thawed D6. Fisher’s exact test. Figure S4. Comparisons of the pregnancy ratio (A) and...

High-resolution figures for Fig. 4 (a)-(d), Fig. 5 (b)(e)(h), Fig.11 (a) (b) and Fig.14 (a)(b)(d)(e), respectively.

Supplemental material, sj-pdf-1-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Supplemental material, sj-pdf-3-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access