83,714 Works

Preparation of Aqueous Extracts from THS 2.2 Aerosol or 3R4F Smoke (Adhesion Assay Study, PMI)

Carine Poussin
This protocol describes the methods used to create aqueous extracts from aerosols or smoke, respectively, that have been used to run an in vitro adhesion assay.

In Vitro Micronucleus genotoxicity assay (Health Canada T-503)

Health Canada
This protocol is the Health Canada Official Method T-503. It describes how to test the total particulate matter from mainstream tobacco smoke on Chinese Hamster Ovary cells using the in vitro Micronucleus Assay.

Multianalyte Profiling of Serum (rodents)

Blaine Phillips
In order to assess inflammatory status, cytokines can be measured in the blood of animals using multianalyte profiling.

Neutral Red Uptake Assay (NRU) in vitro cytotoxicity assay (Health Canada T-502)

Health Canada
The Health Canada T-502 protocol is applicable to the assessment of the cytotoxic potential of cigarette smoke or other types of related aerosols, including reduced-risks products. The method describes the cell preparation and treatment with different fractions of cigarette smoke, the uptake of neutral red dye by untreated and treated cells and the analysis of results.

Bronchoalveolar Lavage Fluid (BALF) Characterization (Apoe-/- mice THS2.2 switching study, PMI)

Blaine Phillips
In order to assess inflammatory status of the lungs, bronchoalveolar lavage fluid can be collected and diverse analyses performed in order to characterize inflammatory cells, proteins, and enzymatic (proteolytic) activity.

Chemical characterization of 58 analytes (PMI list) in aerosols and smoke (Labstat, Health Canada)

Labstat International
The protocol lists analytical methods from Health Canada and Labstat International to detect and quantify major analytes in aerosol and/or smoke. The list of these analytes was defined by PMI as the 58 Harmful or Potentially Harmful Constituents (58 HPHCs), and is divided into 14 analyte groups, plus glycerol.

Coresta Recommended Method n° 38: Determination of reducing carbohydrates in tobacco

CORESTA Scientists
A tobacco extract in 5% acetic acid solution is prepared and the reducing carbohydrates content of the extract is determined by reaction with p-hydroxybenzoic acid hydrazide. In alkaline medium at 85°C, a yellow osazone is formed having an absorption maximum at 410 nm.

Biomarkers of Exposure (Apoe-/- mice THS2.2 switching study, PMI)

Blaine Phillips
Determination of a number of biomarkers of exposure in plasma and urine for in vivo studies.

Aerosol Generation and Analysis of Test Atmospheres (Apoe-/- mice THS2.2 switching study, PMI)

Blaine Phillips
In order to expose in a reproducible manner, the aerosols were generated under specific conditions and well characterized.

Atherosclerotic Plaque Size Measurement (Apoe-/- mice THS2.2 switching study, PMI)

Blaine Phillips
Measurement of atherosclerotic plaque in rodent's aorta by planimetry or microCT.

Transepithelial Electrical Resistance (TEER) Measurement with Chopstick Electrode (in vitro organotypic, PMI)

Stefan Frentzel, Stephanie Johne, Anita Iskandar & Laura Ortega Torres
This protocol describes a measurement of the trans-epithelial electrical resistance (TEER) of 3D organotypic tissues. TEER values indicate tissue integrity and can be measured after cultures are exposed to test substances using the STX2 Chopstick electrode and the EVOM2 Epithelial Voltohmmeter. Using the STX2 electrode, a current is passed through the 3D organotypic tissue via two electrodes. A second pair of electrodes measures the voltage and a resistance value is calculated and displayed by EVOM2...

Transcriptomics profiling from blood samples with Affymetrix microarrays (PMI)

Emmanuel Guedj
This protocol describes a method to analyze the transcriptome of whole blood collected in PAXgene tubes, from RNA purification to network perturbation analysis, using the NuGEN Ovation RNA amplification system and hybridization on Affymetrix microarrays.

Transcriptomics for in vitro 2D cell cultures (PMI)

Emmanuel Guedj & Florian Martin
This protocol describes a method to analyze the transcriptome of cell cultures grown in micro-well plates (2D), from RNA purification to network perturbation analysis, using the NuGEN Ovation® RNA amplification system and hybridization on Affymetrix microarrays.

Statistical Evaluations (Apoe-/- mice THS2.2 switching study, PMI)

Gregory Vuillaume & Patrice Leroy
Summary of statistical methods used for the evaluation of endpoints in the Apoe-/- THS2.2 switching study.

Teeth preparation, in vitro exposure, and coloration assessment

Xiaoyi Zhao, Filippo Zanetti, Shoaib Majeed, Jie Pan, Hans Malmstrom, Manuel Peitsch, Julia Hoeng & Yanfang Ren
This protocol describes the steps necessary to prepare, expose in vitro to an aerosol, and assess the coloration after exposure of the enamel, dentin, and composite repair of teeth.

Proteomics Analysis of Tissues with ITRAQ (Apoe-/- THS switching study, PMI)

Bjorn Titz, Thomas Schneider, Catherine Nury & Ashraf Elamin
Proteomics analysis leveraging the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) method. Computational analysis is also explained.

Proteomics analysis of lung tissue with ITRAQ (rodents, PMI)

Bjorn Titz, Thomas Schneider, Catherine Nury & Ashraf Elamin
This protocol describes how to analyze the proteome of lung tissue with the isobaric Tags for Relative and Absolute Quantification (iTRAQ) methodology, from proteins extraction to data analysis.

PMI's REXC study – Safety variables and other measurements

Christelle Haziza, Guillaume de La Bourdonnaye, Dimitra Skiada, Jacek Ancerewicz, Gizelle Baker, Patrick Picavet & Frank Lüdicke
Safety variables included in this study are: AEs (including device malfunction or misuse), SAEs, medical history and concomitant medications, vital signs (systolic and diastolic blood pressure, pulse rate, respiratory rate), physical examination (including BMI), ECG data, clinical laboratory safety panel (hematology, clinical chemistry, and urine analysis), urine drug screen, alcohol breath test, serological tests, and pregnancy tests.

PMI's PK-05 study - Pharmacokinetic assessment

Patrick Picavet, Christelle Haziza, Nicola Lama, Rolf Weitkunat & Frank Lüdicke
Assessment of pharmacokinetic parameters of nicotine concentration in blood following single use of different test items.

PMI's REXC study – Bioanalytical methods for measurement of biomarkers of exposure

Christelle Haziza, Guillaume de La Bourdonnaye, Dimitra Skiada, Jacek Ancerewicz, Gizelle Baker, Patrick Picavet & Frank Lüdicke
Summarizes methods used to measure biomarkers of exposure in a clinical reduced exposure study with 5 days in a confinement setting (REX-C).

PMI's PK-05 study - Evaluation of subjective effects in clinical study: MCEQ and QSU-brief

Patrick Picavet, Christelle Haziza, Nicola Lama, Rolf Weitkunat & Frank Lüdicke
Questionnaires that can be used to assess the subjective effects of urge-to-smoke (QSU-brief) and nicotine dependence (MCEQ).

PK-05 study (PMI) -Safety variables and measurements

Patrick Picavet, Christelle Haziza, Nicola Lama, Rolf Weitkunat & Frank Lüdicke
Safety parameters measured in the PK05 study.

PK-02 study (PMI) - Pharmacokinetic assessment

Patrick Brossard, Rolf Weitkunat, Valerie Poux, Nicola Lama, Christelle Haziza & Patrick Picavet
Assessment of pharmacokinetic parameters of nicotine concentration in blood following single use of different test items.

PK-02 study (PMI) - Safety variables and measurements

Patrick Brossard, Rolf Weitkunat, Valerie Poux, Nicola Lama, Christelle Haziza & Patrick Picavet
Safety parameters measured in the PK02 study.

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