119 Works

Physical measurements of aerosol/and or smoke (PMI)

Pascal Pratte
The protocol describes the impactor technique used to characterize the particle/droplet size distribution of the aerosol. The physical properties of an aerosol determine the fraction of aerosol that is able to pass through the upper respiratory tract to reach the lungs and the fraction that is retained on the respiratory system.

Aerosol Generation and Analysis of Test Atmospheres (Apoe-/- mice THS2.2 switching study, PMI)

Blaine Phillips
In order to expose in a reproducible manner, the aerosols were generated under specific conditions and well characterized.

Bronchoalveolar Lavage Fluid (BALF) Characterization (Apoe-/- mice THS2.2 switching study, PMI)

Blaine Phillips
In order to assess inflammatory status of the lungs, bronchoalveolar lavage fluid can be collected and diverse analyses performed in order to characterize inflammatory cells, proteins, and enzymatic (proteolytic) activity.

In life observations and body scoring for health monitoring during rodent studies (PMI)

S.K.C. Wong & Blaine Phillips
The protocol describes procedure for the in life observation of animals and body scoring, as part of the health monitoring program during whole body aerosol/smoke exposure rodent studies.

Estimation of the oxidative stress response to aqueous aerosol extracts with the ARE reporter assay (BAT)

Damien Breheny, Jason Adamson, David Azzopardi, Andrew Baxter, Emma Bishop, Tony Carr, Ian Crooks, Katherine Hewitt, Tomasz Jaunky, Sophie Larard, Frazer Lowe, Oluwatobiloba Oke, Mark Taylor, Simone Santopietro, David David, Benjamin Zainuddin, Marianna Gaça, Chuan Liu, James Murphy & Christopher Proctor
This protocol describes the luciferase-based antioxidant response element (ARE) reporter assay for the detection and quantification of Nrf2 transcriptional activity, as a marker of oxidative stress response

Transcriptomics for in vitro 2D cell cultures (PMI)

Emmanuel Guedj & Florian Martin
This protocol describes a method to analyze the transcriptome of cell cultures grown in micro-well plates (2D), from RNA purification to network perturbation analysis, using the NuGEN Ovation® RNA amplification system and hybridization on Affymetrix microarrays.

Characterization of the proteolytic activity in bronchoalveolar lavage fluid (BALF) (PMI)

Blaine Phillips, Patrice Leroy & Gregory Vuillaume
The bronchoalveolar lavage fluid (BALF) collected from mice can be analyzed in order to assess the inflammation status of the lungs after the exposure to toxicants. This protocol describes the methodology required to determine the activity of matrix metalloproteinases (MMPs) in the cell-free BALF supernatant.

Characterization of the free lung cells in bronchoalveolar lavage fluid (BALF, PMI)

Blaine Phillips, Patrice Leroy & Gregory Vuillaume
The bronchoalveolar lavage fluid (BALF) collected from mice or rats can be analyzed in order to assess the inflammation status of the lungs after the exposure to toxicants. This protocol describes the methodology required to characterize the different cell subpopulations contained in the BALF.

Vasculature on-a-chip model for testing microvessel adhesion under flow (PMI)

Carine Poussin, Bart Kramer, Henriette Lanz, Angelique Van der Heuvel, Alexandra Laurent, Thomas Olivier, Marjolein Vermeer, Dariusz Peric, Karine Baumer, Rémi Dulize & Emmanuel Guedj
The protocol describes the preparation of monocyte-endothelial cell cultures to create a vasculature on-a-chip model using the OrganoPlate® system, including barrier integrity assay, and the testing of microvessel adhesion under flow after exposure to media conditioned with 3R4F/THS 2.2 or other stimulant.

Parallel-reaction monitoring (PRM)

Thomas Schneider
This protocol is used for parallel reaction monitoring (PRM)-based targeted mass spectrometry.

Liver Histopathology and Histomorphometry (rodents, PMI)

Blaine Phillips & Ee Tsin Wong
Histopathology and histomorphometry characterization of liver tissue from rodents involved in inhalation toxicology studies

mRNA Analysis for in vitro organotypic culture (incl. data analysis, PMI)

Emmanuel Guedj, Karine Baumer, Remi Dulize, Florian Martin, Alain Sewer & Yang Xiang
Analysis of the transcriptome of 3D organotypic tissue cultures with Affymetrix microarrays, starting from RNA purification until network perturbation analysis.

mRNA Analysis (rodents, PMI)

Blaine Phillips, Emmanuel Guedj, Karine Baumer, Remi Dulize, Florian Martin, Alain Sewer & Yang Xiang
Summary of transcriptomics analysis for various tissues collected in an inhalation study in mice, from sample collection to biological interpretation and network perturbation analysis.

Measurement of deposited particulate mass and nicotine at the exposure interface (BAT)

Damien Breheny, Jason Adamson, David Azzopardi, Andrew Baxter, Emma Bishop, Tony Carr, Ian Crooks, Katherine Hewitt, Tomasz Jaunky, Sophie Larard, Frazer Lowe, Oluwatobiloba Oke, Mark Taylor, Simone Santopietro, David David, Benjamin Zainuddin, Marianna Gaça, Chuan Liu, James Murphy & Christopher Proctor
This protocol describes how to obtain deposition data by assessment of whole aerosol particulate masses at the exposure interface.

Composite resins preparation and assessment

Xiaoyi Zhao, Filippo Zanetti, Shoaib Majeed, Jie Pan, Hans Malmstrom, Manuel Peitsch, Julia Hoeng & Yanfang Ren
This protocol describes the steps necessary to prepare composite resins discs and to assess their color, gloss, and surface roughness.

Transcriptomics profiling from blood samples with Affymetrix microarrays (PMI)

Emmanuel Guedj
This protocol describes a method to analyze the transcriptome of whole blood collected in PAXgene tubes, from RNA purification to network perturbation analysis, using the NuGEN Ovation RNA amplification system and hybridization on Affymetrix microarrays.

Shotgun lipidomics analysis of mouse lung tissues (PMI)

Oksana Lavrynenko, Sophie Dijon & Bjorn Titz
This protocol describes how to determine the lipidomics profile of mouse lung cryosections using BUME extraction followed by shotgun analysis.

Analysis of indoor air constituents: RSP, UVPM, FPM and Solanesol (PMI)

Maya Mitova, Catherine Goujon-Ginglinger & Camille Cluse
The protocol describes the quantification of Respirable Suspended Particles (RSP), ultraviolet particulate matter (UVPM), fluorescent particulate matter (FPM), and solanesol that are marker constituents in Environmental Tobacco Smoke (ETS).

Determination of eight carbonyls in aerosols trapped in PBS (PMI)

Daniela Correia, Jannio Albino, Shoaib Majeed & Audrey Debrick
The protocol describes the procedure of trapping carbonyls in phosphate buffered saline (PBS) and the quantification in PBS solutions of formaldehyde, acetaldehyde, acetone, acrolein, crotonaldehyde, propionaldehyde, methyl ethyl ketone (butan-2-one, MEK), and butyraldehyde after derivation, using HPLC-MS/MS with electrospray ionization (ESI) interface in negative ionization mode.

Quantification of reactive oxygen species generated in response to aqueous aerosol extracts with the DCF assay (BAT)

Damien Breheny, Jason Adamson, David Azzopardi, Andrew Baxter, Emma Bishop, Tony Carr, Ian Crooks, Katherine Hewitt, Tomasz Jaunky, Sophie Larard, Frazer Lowe, Oluwatobiloba Oke, Mark Taylor, Simone Santopietro, David David, Benjamin Zainuddin, Marianna Gaça, Chuan Liu, James Murphy & Christopher Proctor
This protocol describes the assessment by the DCF assay of intracellular oxidative stress– more specifically of the increase in reactive oxygen species – in response to aqueous aerosol extracts.

Analysis of whole aerosol emissions (BAT)

Jennifer Margham
This protocol describes how to quantify aerosol composition from different products using twenty-seven analytical methods to quantify the emissions of 150 different measurands (142 analytes and 8 collated values) in the mainstream aerosols of cigarettes or eCigarettes (Extracted from (Margham et al., 2016)). Margham, J. et al. Chemical Composition of Aerosol from an E-Cigarette: A Quantitative Comparison with Cigarette Smoke. Chem Res Toxicol 29, 1662-1678, doi:10.1021/acs.chemrestox.6b00188 (2016).

Analysis of indoor air constituents: tobacco specific nitrosamines NNN and NNK (PMI)

Mathieu Schaller & Michel Rotach
The protocol describes the method of quantification of two tobacco specific nitrosamines, N-Nitrosonornicotine (NNN), and 4-(methylnitrosamino)-1-(3-pyridil)-1-butanone (NNK) in air samples collected in a dedicated Indoor Air Quality room (IAQ-room) by LC-MS/MS analysis.

PMI's REXC study – Bioanalytical methods for measurement of biomarkers of exposure

Christelle Haziza, Guillaume de La Bourdonnaye, Dimitra Skiada, Jacek Ancerewicz, Gizelle Baker, Patrick Picavet & Frank Lüdicke
Summarizes methods used to measure biomarkers of exposure in a clinical reduced exposure study with 5 days in a confinement setting (REX-C).

Liver Histopathology and Histomorphometry (rodents, PMI)

Blaine Phillips
Histopathology and histomorphometry characterization of liver tissue from rodents involved in inhalation toxicology studies

Proteomics analysis of lung tissue with ITRAQ (rodents, PMI)

Bjorn Titz, Thomas Schneider, Catherine Nury & Ashraf Elamin
This protocol describes how to analyze the proteome of lung tissue with the isobaric Tags for Relative and Absolute Quantification (iTRAQ) methodology, from proteins extraction to data analysis.

Registration Year

  • 2019
    119

Resource Types

  • Workflow
    119

Data Centers

  • INTERVALS
    119